CAJ | 학술논문

目的：探讨新型 B 族链球菌（GBS）胶体金免疫层析试纸条的临床检测效果。方法选取202份女性的阴道或宫颈棉拭子标本，采用新型检测试剂与对照试剂作对比，进行临床应用评价。结果新型试剂最低检出浓度为105 CFU／ml，5～8 min 内即可观察结果；与对照试剂相比，考核试剂灵敏度更好，显色时间更短；检测的202份临床标本中，与对照试剂相比，197例样本检测结果一致，其余5例样本检测结果不符，阳性符合率达97.5％，阴性符合率达97.54％，总符合率为97.52％，Kappa 值0.948，一致性分析表明新型试剂与对照试剂具有很好的一致性。结论该新型试剂能有效的辅助诊断 B 族链球菌感染，具有较高的临床应用价值。
목적：탐토신형 B 족련구균（GBS）효체금면역층석시지조적림상검측효과。방법선취202빈녀성적음도혹궁경면식자표본，채용신형검측시제여대조시제작대비，진행림상응용평개。결과신형시제최저검출농도위105 CFU／ml，5～8 min 내즉가관찰결과；여대조시제상비，고핵시제령민도경호，현색시간경단；검측적202빈림상표본중，여대조시제상비，197례양본검측결과일치，기여5례양본검측결과불부，양성부합솔체97.5％，음성부합솔체97.54％，총부합솔위97.52％，Kappa 치0.948，일치성분석표명신형시제여대조시제구유흔호적일치성。결론해신형시제능유효적보조진단 B 족련구균감염，구유교고적림상응용개치。
Objective To explore the effect of a novel colloidal gold immunochromatographic test strip for detection of group B streptococci (GBS).Methods A total of 202 cases of swab of vagina or neck of uterus were collected,and they were detec-ted by novel strip and control strip to evaluate their clinical applications.Results Sensitivity of novel strip was about 105 CFU/ml and the detection time was about 5 to 8 minutes,and it showed better sensitivity and shorter detection time com-pared with control strip.In the 202 cases of clinical samples,the detection results of 197 cases were in consistent with the control strip,however,the detection results of 5 cases were not in consistent.The positive coincidence rate and negative coin-cidence rate were 97.5% and 97.54% respectively,and the total coincidence rate and Kappa value were 97.52% and 0.948 respectively.The consistency test showed no significant difference between this strip and control strip.Conclusion This method was a effective technology for diagnosing of infection caused by GBS,and had high value in clinical application.