国际眼科杂志
國際眼科雜誌
국제안과잡지
International Eye Science
2015年
10期
1800-1801
,共2页
阎艳%武鹏安%刘先宁%王东岳%张仙娇%崔家瑞
閻豔%武鵬安%劉先寧%王東嶽%張仙嬌%崔傢瑞
염염%무붕안%류선저%왕동악%장선교%최가서
真菌性角膜炎%石蜡切片%异硫氰酸荧光素染色法%过碘酸雪夫氏染色法
真菌性角膜炎%石蠟切片%異硫氰痠熒光素染色法%過碘痠雪伕氏染色法
진균성각막염%석사절편%이류청산형광소염색법%과전산설부씨염색법
fungal keratitis%paraffin sections%fluorescein isothiocyanate staining%periodic acid -Schiff staining
目的：寻找真菌性角膜炎组织切片快速、准确、方便操作的特殊染色方式。<br> 方法：采用经真菌培养均为阳性的真菌性角膜炎患者的角膜标本38例，常规固定脱水透明浸蜡，进行4μm石蜡切片，脱蜡至水，选用异硫氰酸荧光素( FITC )荧光法和过碘酸雪夫氏染色法( PAS)进行染色对比。<br> 结果：异硫氰酸荧光素法检测阳性率为97.4%(37/38)，过碘酸雪夫氏染色法检测阳性率为86.8%(33/38)。结果比较有统计学差异(P<0.001)。<br> 结论：异硫氰酸荧光素法镜检真菌性角膜炎优于过碘酸雪夫氏染色法镜检。
目的：尋找真菌性角膜炎組織切片快速、準確、方便操作的特殊染色方式。<br> 方法：採用經真菌培養均為暘性的真菌性角膜炎患者的角膜標本38例，常規固定脫水透明浸蠟，進行4μm石蠟切片，脫蠟至水，選用異硫氰痠熒光素( FITC )熒光法和過碘痠雪伕氏染色法( PAS)進行染色對比。<br> 結果：異硫氰痠熒光素法檢測暘性率為97.4%(37/38)，過碘痠雪伕氏染色法檢測暘性率為86.8%(33/38)。結果比較有統計學差異(P<0.001)。<br> 結論：異硫氰痠熒光素法鏡檢真菌性角膜炎優于過碘痠雪伕氏染色法鏡檢。
목적：심조진균성각막염조직절편쾌속、준학、방편조작적특수염색방식。<br> 방법：채용경진균배양균위양성적진균성각막염환자적각막표본38례，상규고정탈수투명침사，진행4μm석사절편，탈사지수，선용이류청산형광소( FITC )형광법화과전산설부씨염색법( PAS)진행염색대비。<br> 결과：이류청산형광소법검측양성솔위97.4%(37/38)，과전산설부씨염색법검측양성솔위86.8%(33/38)。결과비교유통계학차이(P<0.001)。<br> 결론：이류청산형광소법경검진균성각막염우우과전산설부씨염색법경검。
AIM: To find special staining methods which is fast, accurate and easy to operate for fungal keratitis tissue section. <br> METHODS:Thirty- eight cases with fungal keratitis were diagnosed by positive fungal culture. Corneal specimens of these cases were conventionally fixed, dehydrated and dipped in wax for 4μm paraffin sections, then subject to conventional dewaxing to water. The results of fluorescein isothiocyanate ( FITC) and periodic acid-Schiff ( PAS) staining were compared. <br> RESULTS: Positive detection rate of FITC staining was 97. 4% (37/38), positive detection rate of PAS staining was 86. 8% ( 33/38 ), with statistically significant difference (P<0. 001). <br> CONCLUSION: FITC staining method is simple, fast, accurate, andhas high positive rate. It is superior to PAS staining, and is worth of promoting.
