CAJ | 학술논문

以含32个碱基的聚脱氧腺苷酸( A32)作为黏合剂，将水合肼还原的石墨烯( RGO)固定在金( Au)电极表面，制备A32/RGO纳米复合膜电化学传感器.利用循环伏安法和交流阻抗法对传感器制备过程进行表征，并用循环伏安法和微分脉冲伏安法研究了该传感器的电化学行为.研究结果表明，与裸Au电极和A32修饰的Au电极相比， RGO/A32/Au电极对核黄素有较高的电化学活性，其还原峰电流与核黄素的浓度在0.025~2.75μmol/L范围内呈线性关系，检出限(S/N=3)为15 nmol/L.该传感器具有灵敏度高、抗干扰能力强及稳定性好等优点，可用于人尿中核黄素的分析测定.
이함32개감기적취탈양선감산( A32)작위점합제，장수합정환원적석묵희( RGO)고정재금( Au)전겁표면，제비A32/RGO납미복합막전화학전감기.이용순배복안법화교류조항법대전감기제비과정진행표정，병용순배복안법화미분맥충복안법연구료해전감기적전화학행위.연구결과표명，여라Au전겁화A32수식적Au전겁상비， RGO/A32/Au전겁대핵황소유교고적전화학활성，기환원봉전류여핵황소적농도재0.025~2.75μmol/L범위내정선성관계，검출한(S/N=3)위15 nmol/L.해전감기구유령민도고、항간우능력강급은정성호등우점，가용우인뇨중핵황소적분석측정.
A novel electrochemical sensor to detect riboflavin was constructed using 32-mer polydeoxyadenylic acid( A32 ) as an effective adhesive for binding with the hydrazine hydrate-reduced graphene oxide( RGO) and the gold( Au) electrode. The fabrication process of the sensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy, and its electrochemical behaviour was studied by cyclic voltammetry and differential pulse voltammetry. The experimental results showed that compared with the bare Au electrode and the A32/Au electrode, the RGO/A32/Au electrode displayed excellent electrochemical activity towards riboflavin, and the cathodic peak current increased linearly with the increasing concentration of riboflavin from 0. 025 μmol/L to 2. 75 μmol/L with a low detection limit of 15 nmol/L(S/N=3). The sensor possesses high sensitivity, strong anti-interference ability and good stability, and can be used for the analysis of riboflavin in human urine samples.