CAJ | 학술논문

目的：了解北京地区人类巨细胞病毒（HCMV）及人类 T 淋巴细胞白血病病毒（HTLV）在无偿献血人群中的感染情况。方法北京地区2010例无偿献血者血液标本采用 ELISA 方法筛查 HCMV-IgG，HCMV-IgM，HTLV-Ⅰ／Ⅱ抗体。初检阳性标本进行双孔复检，两次检测结果均为阳性标本判定为 ELISA 结果阳性，HTLV 阳性样品经巢式 PCR 检测核酸进行确认。结果北京地区2010例无偿献血者中 HCMV-IgM 和 HCMV-IgG 阳性率分别为2.19％和92.59％，ELISA法初筛抗-HTLV 阳性1例，巢式 PCR 确认结果阴性。结果通过χ2检验统计学分析显示，无偿献血者 HCMV-IgG 阳性率男性低于女性（χ2＝5.88，P ＜0.05），18～25岁年龄段的献血者 HCMV-IgG 和 HCMV-IgM 阳性率低于其他年龄段（χ2＝16.51，21.52；均 P ＜0.05），大学生 HCMV-IgG 阳性率低于其他职业献血者（χ2＝14.20，P ＜0.05），而献血者教育程度与 HCMV-IgG 和 HCMV-IgM 阳性率无关（P ＞0.05）。结论在该次调查中，北京地区2010例无偿献血者中未发现感染HTLV 的病例，而 HCMV 感染率与性别、年龄、职业相关，与教育程度无关。
목적：료해북경지구인류거세포병독（HCMV）급인류 T 림파세포백혈병병독（HTLV）재무상헌혈인군중적감염정황。방법북경지구2010례무상헌혈자혈액표본채용 ELISA 방법사사 HCMV-IgG，HCMV-IgM，HTLV-Ⅰ／Ⅱ항체。초검양성표본진행쌍공복검，량차검측결과균위양성표본판정위 ELISA 결과양성，HTLV 양성양품경소식 PCR 검측핵산진행학인。결과북경지구2010례무상헌혈자중 HCMV-IgM 화 HCMV-IgG 양성솔분별위2.19％화92.59％，ELISA법초사항-HTLV 양성1례，소식 PCR 학인결과음성。결과통과χ2검험통계학분석현시，무상헌혈자 HCMV-IgG 양성솔남성저우녀성（χ2＝5.88，P ＜0.05），18～25세년령단적헌혈자 HCMV-IgG 화 HCMV-IgM 양성솔저우기타년령단（χ2＝16.51，21.52；균 P ＜0.05），대학생 HCMV-IgG 양성솔저우기타직업헌혈자（χ2＝14.20，P ＜0.05），이헌혈자교육정도여 HCMV-IgG 화 HCMV-IgM 양성솔무관（P ＞0.05）。결론재해차조사중，북경지구2010례무상헌혈자중미발현감염HTLV 적병례，이 HCMV 감염솔여성별、년령、직업상관，여교육정도무관。
Objective To analysis the human cytomegalovirus (HCMV)and human T lymphotropic virus(HTLV)infection status in Beijing among voluntary blood donors.Methods Randomly selected 2 010 blood samples from five districts and counties of Beijing City to screen HCMV-IgG,HCMV-IgM and HTLV-Ⅰ/Ⅱ antibody by ELISA method.The positive samples were reexamined two times,two test results of samples were positive that were determined positive by ELISA. HTLV positive samples was confirmed by nested PCR.Results The HCMV-IgM and HCMV-IgG positive rates of Beijing blood donors were 2.19% and 92.59%,screened 1 case of anti-HTLV positive by ELISA method,then confirmed to be neg-ative result by nested PCR.The statistics showed that the HCMV-IgG positive rate female blood donors was higher than male (P <0.05).The positive rates of HCMV-IgM and HCMV-IgG among18~25 years old donors was lowest (P <0.05). Positive rate of HCMV-IgG in college students was lower than other occupation blood donars (P <0.05)and education de-gree was independent of HCMV-IgG,HCMV-IgM positive rates (P >0.05).Conclusion In this investigation,2 010 cases of voluntary blood donors from five districts of Beijing were not found in cases of HTLV infection,HCMV infection was prevalent.