CAJ | 학술논문

目的探讨足细胞骨架蛋白Synaptopodin在高脂饮食所致肾发育期足细胞损伤中的表达及意义.方法 32只3周龄雄性C57BL/6J幼鼠随机分为正常饮食组和高脂饮食组,分别给予标准饲料与高脂饲料喂养4周.检测葡萄糖耐量水平、尿微量白蛋白与尿肌酐;观察小鼠肾的病理形态学改变,应用免疫组织化学和免疫印迹技术检测小鼠肾足细胞中Synaptopodin的表达变化.结果与正常饮食组相比,高脂饮食组小鼠体质量显著增加,葡萄糖调节受损,尿微量白蛋白与尿肌酐比率增高.镜下可见肾小球肥大,肾小囊腔内渗出,系膜基质轻度增生等病理改变.同时足细胞Synaptopodin的表达明显降低.结论 Synaptopodin在高脂饮食诱导的C57BL/6J幼鼠肾足细胞中表达显著降低,并出现蛋白尿.
목적 탐토족세포골가단백Synaptopodin재고지음식소치신발육기족세포손상중적표체급의의.방법 32지3주령웅성C57BL/6J유서수궤분위정상음식조화고지음식조,분별급여표준사료여고지사료위양4주.검측포도당내량수평、뇨미량백단백여뇨기항;관찰소서신적병리형태학개변,응용면역조직화학화면역인적기술검측소서신족세포중Synaptopodin적표체변화.결과 여정상음식조상비,고지음식조소서체질량현저증가,포도당조절수손,뇨미량백단백여뇨기항비솔증고.경하가견신소구비대,신소낭강내삼출,계막기질경도증생등병리개변.동시족세포Synaptopodin적표체명현강저.결론 Synaptopodin재고지음식유도적C57BL/6J유서신족세포중표체현저강저,병출현단백뇨.
Objective To investigate the expression and significance of Synaptopodin resulting in podocyte injury in high-fat diet C57BL/6J mice.Methods Thirty-two male C57BL/6J mice were randomly divided into two groups. The standard group was fed with basic diet and high-fat diet group was fed with high-fat diet for 4 weeks. Body weight and urine protein changes were measured. Insulin resistance was detected by glucose tolerance test. The kidney tissue morphological changes were examined by HE staining. The expression of Synaptopodin was examined by immunohistochemistry and western blotting.Results In high-fat diet group, the body weight was higher than the standard group, and therewas significant decrease in glucose tolerance levels and rise in urinary albumin/ creatinine ratio. HE staining results showed that there were glomeruli hypertrophy, mesangial matrix hyperplasia, and glomerulosclerosis. Compared with standard group, the expression of Synaptopodin in high-fat diet group reduced significantly in podocyte.Conclusion The expression of Synaptopodin in podocyte of high-fat diet C57BL/6J mice reduces significantly, which may lead to proteinuria.