CAJ | 학술논문

目的了解睑脂的种类和成分,并分析比较健康者和睑板腺功能障碍(MGD)患者的睑脂成分.方法病例对照研究.正常组20例,MGD组19例,均采用改良的睑板腺压榨方法收集睑脂,采用GC-MS法分析睑脂成分与结构.条件:色谱柱为DB-5 MS(30 m×0.25 mmX0.25 μm)毛细管柱,载气为He,流速1.0 ml/min;进样量:1 ml.质谱条件:质谱电离方式为电子电离(EI),电子轰击源电子能量70 eV,离子源温度200℃;电子倍增器电压1.10 kV;溶剂延迟时间:1.00 min;扫描方式:san,扫描范围m/z:20～500 aum.通过E1质谱碎片和数据库中所存储的标准物质的质谱信息的比对的方式进行鉴定.采用色谱峰面积归一化法,计算其组分的相对含量.各睑脂成分相对含量数据用中位数和四分位数间距表示,采用非参数检验进行统计学比较.结果通过GC-MS系统,共分离出102种睑脂成分,明确鉴定23种化合物,包括3种烷烃、4种烯、2种醇、1种芳烃、7种酯、4种脂肪酸、1种酰胺、1种酚,尚有79个色谱峰因为质谱图库中无相应物质信息,未进行对比鉴定.睑脂成分的个体差异性较大.其中软脂酸、硬脂酸、角鲨烯水平在MGD患者组明显低于正常组(U软脂酸=-2.086,U硬脂酸-2.027,U角鲨烯=-2.866;P＜0.05).结论睑脂成分种类繁多,结构复杂,个体差异大,其中软脂酸、硬脂酸、角鲨烯在MGD患者中明显降低,提示MGD与睑脂成分改变有关.
목적 료해검지적충류화성분,병분석비교건강자화검판선공능장애(MGD)환자적검지성분.방법 병례대조연구.정상조20례,MGD조19례,균채용개량적검판선압자방법수집검지,채용GC-MS법분석검지성분여결구.조건:색보주위DB-5 MS(30 m×0.25 mmX0.25 μm)모세관주,재기위He,류속1.0 ml/min;진양량:1 ml.질보조건:질보전리방식위전자전리(EI),전자굉격원전자능량70 eV,리자원온도200℃;전자배증기전압1.10 kV;용제연지시간:1.00 min;소묘방식:san,소묘범위m/z:20～500 aum.통과E1질보쇄편화수거고중소존저적표준물질적질보신식적비대적방식진행감정.채용색보봉면적귀일화법,계산기조분적상대함량.각검지성분상대함량수거용중위수화사분위수간거표시,채용비삼수검험진행통계학비교.결과 통과GC-MS계통,공분리출102충검지성분,명학감정23충화합물,포괄3충완경、4충희、2충순、1충방경、7충지、4충지방산、1충선알、1충분,상유79개색보봉인위질보도고중무상응물질신식,미진행대비감정.검지성분적개체차이성교대.기중연지산、경지산、각사희수평재MGD환자조명현저우정상조(U연지산=-2.086,U경지산-2.027,U각사희=-2.866;P＜0.05).결론 검지성분충류번다,결구복잡,개체차이대,기중연지산、경지산、각사희재MGD환자중명현강저,제시MGD여검지성분개변유관.
Objective To analyze and identify the meibum from healthy individuals and patients with meibomian gland dysfunction (MGD) by gas chromatography-mass spectrometry (GC-MS).Methods Case-control study.The meibum from twenty healthy persons and nineteen MGD patients was separated and analyzed by GC-MS.GC conditions were:column:DB-5 MS (30 m × 0.25 mm × 0.25 μm) capillary column,carrier gas:He,flow rate:1.0 ml/min,injection amount:1 ml.MS conditions were:MS ionization mode:EI,electron impact source electron energy:70 eV,ion source temperature:200 ℃,electron multiplier voltage of 1.10 kV,solvent delay time:1.00 min,scanning mode:San,scanning range m/z 20-500 aum.The components of meibum were identified by E1 mass fragmentation and the standard substance in the database.The relative content of the components was calculated by the method of chromatographic peak area normalization.Results In the 39 samples that were analyzed by GC-MS,102 chromatography peaks were separated,and 23 compounds were identified,including 3 alkanes,4 alkenes,2 alcohols,1 aromatic hydrocarbon,7 esters,4 fatty acids,1 amide,and 1 phenol.However,there was no corresponding physical mass library information in the remaining 79 GC peaks,and no identification could be performed.The relative concentration level of palmitic acid,stearic acid,and squalene in the MGD group was significantly lower than that in the healthy controls (U=-2.086,-2.027,-2.866,P＜0.05).Conclusions Meibum has a wide variety of components,the structures are complex,and the components in different individuals vary greatly.Palmitic acid,stearic acid,and squalene levels were significantly decreased in MGD patients compared to the healthy group,suggesting that MGD is related to changes of the meibum components.