应用化工
應用化工
응용화공
Applied Chemical Industry
2015年
9期
1621-1626
,共6页
谷耀华%薛屏%李鹏%夏维涛
穀耀華%薛屏%李鵬%夏維濤
곡요화%설병%리붕%하유도
固定化假单胞菌脂肪酶%功能化介孔载体%( R,S)-3-羟基丁酸乙酯%转酯化拆分
固定化假單胞菌脂肪酶%功能化介孔載體%( R,S)-3-羥基丁痠乙酯%轉酯化拆分
고정화가단포균지방매%공능화개공재체%( R,S)-3-간기정산을지%전지화탁분
immobilized Pseudomonas sp lipase%functionalized mesoporous support%( R,S )-ethyl-3-hydroxybutyrate%transesterification resolution
设计制备出比表面积290.6 m2/g、平均孔径和孔体积16.5 nm和1.66 cm3的功能化介孔载体G-NH2-MCF, G-NH2-MCF共价结合假单胞菌脂肪酶( Pseudomonas sp Lipase,PSL),获得了固定化酶PSL/G-NH2-MCF。在甲苯溶剂中,乙酸乙烯酯作酰化剂,固定化酶PSL/G-NH2-MCF催化( R,S)-3-羟基丁酸乙酯的转酯化拆分反应,转化率为15.6%,(S)-3-羟基丁酸乙酯的对映体过量值(eeS)为17.8%,(R)-3-乙酸基丁酸乙酯对映体过量值(eeP)为96.2%,对映选择性参数E为61；而在相同条件下,使用游离酶PSL催化拆分反应,转化率、eeS、eeP和E值分别为2.16%,1.95%,88.2%和16,PSL经G-NH2-MCF固定化后催化性能得到明显提高。同时研究了固定化酶PSL/G-NH2-MCF催化( R,S)-3-羟基丁酸乙酯转酯拆分的反应条件对转化率和对映选择性的影响规律。
設計製備齣比錶麵積290.6 m2/g、平均孔徑和孔體積16.5 nm和1.66 cm3的功能化介孔載體G-NH2-MCF, G-NH2-MCF共價結閤假單胞菌脂肪酶( Pseudomonas sp Lipase,PSL),穫得瞭固定化酶PSL/G-NH2-MCF。在甲苯溶劑中,乙痠乙烯酯作酰化劑,固定化酶PSL/G-NH2-MCF催化( R,S)-3-羥基丁痠乙酯的轉酯化拆分反應,轉化率為15.6%,(S)-3-羥基丁痠乙酯的對映體過量值(eeS)為17.8%,(R)-3-乙痠基丁痠乙酯對映體過量值(eeP)為96.2%,對映選擇性參數E為61；而在相同條件下,使用遊離酶PSL催化拆分反應,轉化率、eeS、eeP和E值分彆為2.16%,1.95%,88.2%和16,PSL經G-NH2-MCF固定化後催化性能得到明顯提高。同時研究瞭固定化酶PSL/G-NH2-MCF催化( R,S)-3-羥基丁痠乙酯轉酯拆分的反應條件對轉化率和對映選擇性的影響規律。
설계제비출비표면적290.6 m2/g、평균공경화공체적16.5 nm화1.66 cm3적공능화개공재체G-NH2-MCF, G-NH2-MCF공개결합가단포균지방매( Pseudomonas sp Lipase,PSL),획득료고정화매PSL/G-NH2-MCF。재갑분용제중,을산을희지작선화제,고정화매PSL/G-NH2-MCF최화( R,S)-3-간기정산을지적전지화탁분반응,전화솔위15.6%,(S)-3-간기정산을지적대영체과량치(eeS)위17.8%,(R)-3-을산기정산을지대영체과량치(eeP)위96.2%,대영선택성삼수E위61；이재상동조건하,사용유리매PSL최화탁분반응,전화솔、eeS、eeP화E치분별위2.16%,1.95%,88.2%화16,PSL경G-NH2-MCF고정화후최화성능득도명현제고。동시연구료고정화매PSL/G-NH2-MCF최화( R,S)-3-간기정산을지전지탁분적반응조건대전화솔화대영선택성적영향규률。
Functionalized mesoporous support G-NH2-MCF with specific surface area of 290. 6 m2/g,av-erage pore diameter of 16. 5 nm and pore volume of 1. 66 cm3 was prepared. The Pseudomonas sp lipase PSL was immobilized covalently on G-NH2-MCF materials to prepare the immobilized enzyme PSL/G-NH2-MCF. The PSL/G-NH2-MCF catalyzed transesterification resolutionin of ( R,S)-ethyl-3-hydroxybu-tyrate with vinyl acetate as acylation agent in toluene,17. 8% enantiomeric excess (eeS) of (S)-ethyl-3-hydroxybutyrate,96. 2% enantiomeric excess (eeP) of (R)-ethyl-3-acetroxybutyrate,and conversion of 15. 6% with 61 enantiomeric ratio were obtained. While using free PSL in the resolution reaction under the same conditions,the eeS,eeP,the conversion and enantiomeric ratio E were 1. 95%,88. 2%,2. 16%and 16,respectively,which means that the activity and enantioselectivity of PSL are improved significantly by the immobilization on G-NH2-MCF. The effects of the reaction conditions on the conversion and enanti-oselectivity of PSL/G-NH2-MCF for the transesterification resolutionin were studied.
