CAJ | 학술논문

本文以孕穗期的杂交粳稻花优14及其母本申9A和父本繁14的剑叶为材料，利用Affymetrix水稻基因组芯片检测了3个样品中的基因表达谱，并用生物信息学方法对差异表达基因进行了分析。结果表明：与其亲本相比，杂交粳稻花优14中共有2057个基因的表达水平出现了2倍(变化倍数≥2或≤0.5)以上的变化。通过对这些差异表达基因进行GO(Gene Ontology)功能分类，发现差异表达基因在光合系统Ⅰ、叶绿体膜和叶绿体被膜等与叶绿体相关的细胞组分中显著富集；同时差异表达基因还在叶绿素合成、叶绿素代谢和类胡萝卜素合成等生物学过程中富集。光合作用效率的改变可能和花优14杂种优势的形成相关。与已报道结果不同，本文在代谢途径分析结果中并没有发现花优14中差异表达基因在碳固定和光合作用等途径中显著的富集，但是发现差异表达基因在光合作用?天线蛋白以及淀粉和蔗糖的代谢途径中显著富集。该结果表明，在不同的杂交组合中，参与杂种优势形成的基因或者代谢途径可能是不同的。
본문이잉수기적잡교갱도화우14급기모본신9A화부본번14적검협위재료，이용Affymetrix수도기인조심편검측료3개양품중적기인표체보，병용생물신식학방법대차이표체기인진행료분석。결과표명：여기친본상비，잡교갱도화우14중공유2057개기인적표체수평출현료2배(변화배수≥2혹≤0.5)이상적변화。통과대저사차이표체기인진행GO(Gene Ontology)공능분류，발현차이표체기인재광합계통Ⅰ、협록체막화협록체피막등여협록체상관적세포조분중현저부집；동시차이표체기인환재협록소합성、협록소대사화류호라복소합성등생물학과정중부집。광합작용효솔적개변가능화화우14잡충우세적형성상관。여이보도결과불동，본문재대사도경분석결과중병몰유발현화우14중차이표체기인재탄고정화광합작용등도경중현저적부집，단시발현차이표체기인재광합작용?천선단백이급정분화자당적대사도경중현저부집。해결과표명，재불동적잡교조합중，삼여잡충우세형성적기인혹자대사도경가능시불동적。
Gene expression profiling using microarray has contributed significantly to heterosis studies. Using the Affymetrix rice genome array, we investigated gene expression profiles in the flag leaves of thejaponica hybrid rice Huayou14 and its parental cultivars Shen9A and Fan14 at the booting stage. A total of 2057 genes differentially ex-pressed (fold change≥2 or≤0.5) between Huayou14 and its parents were identified. Functional classification of the differentially expressed genes by Gene Ontology (GO) analysis indicated the differentially expressed genes were significantly enriched in photosynthesis-related cellular component categories (e.g. photosystemⅠ, chloroplast membrane and chloroplast envelope), and biological process categories (e.g. chlorophyll catabolic, chlorophyll bio-synthetic and carotenoid biosynthetic processes). These results suggest that the changes in the photosynthetic ability of thejaponica hybrid rice Huayou14 may be related to heterosis. Metabolic pathway analysis indicated that differen-tially expressed genes were significantly enriched in photosynthesis-antenna proteins and starch and sucrose meta-bolic pathways, instead of photosynthesis and carbon fixation pathways as reported previously. These results suggest that different genes or metabolic pathways might contribute to the heterosis of different hybrid combinations.