外科研究与新技术
外科研究與新技術
외과연구여신기술
Surgical research and new technique
2015年
2期
114-122
,共9页
3D培养支架%肝细胞诱导分化%干细胞
3D培養支架%肝細胞誘導分化%榦細胞
3D배양지가%간세포유도분화%간세포
3D culture scaffolds%Hepatic differentiation%Stem cells
功能性肝细胞被广泛应用于细胞移植、生物人工肝治疗、肝脏组织工程、药物代谢及毒性等研究,但作为功能性肝细胞主要来源之一的原代肝细胞无法完全满足这些研究需求.在2D或3D细胞培养体系中经干细胞诱导获得的功能性肝细胞可能可作为替代来源.目前,3D细胞诱导体系与2D体系相比,因前者能模拟体内部分生物微环境而备受关注.该文概述3D细胞培养体系在促进干细胞向肝细胞诱导分化研究中的作用进展,重点关注用于研制3D培养支架的材料.
功能性肝細胞被廣汎應用于細胞移植、生物人工肝治療、肝髒組織工程、藥物代謝及毒性等研究,但作為功能性肝細胞主要來源之一的原代肝細胞無法完全滿足這些研究需求.在2D或3D細胞培養體繫中經榦細胞誘導穫得的功能性肝細胞可能可作為替代來源.目前,3D細胞誘導體繫與2D體繫相比,因前者能模擬體內部分生物微環境而備受關註.該文概述3D細胞培養體繫在促進榦細胞嚮肝細胞誘導分化研究中的作用進展,重點關註用于研製3D培養支架的材料.
공능성간세포피엄범응용우세포이식、생물인공간치료、간장조직공정、약물대사급독성등연구,단작위공능성간세포주요래원지일적원대간세포무법완전만족저사연구수구.재2D혹3D세포배양체계중경간세포유도획득적공능성간세포가능가작위체대래원.목전,3D세포유도체계여2D체계상비,인전자능모의체내부분생물미배경이비수관주.해문개술3D세포배양체계재촉진간세포향간세포유도분화연구중적작용진전,중점관주용우연제3D배양지가적재료.
Functional hepatocytes have been used in researches on cell transplantation,bio-artificial liver therapy, liver tissue engineering,drug metabolism and toxicity. Primary hepatocytes (PHs),as a source of functional hepatocytes, cannot meet the growing demands in those researches. Functional hepatocytes differentiated in 2D or 3D culture systems from stem cells are considered an alternative cell source. 3D culture systems,mimicking the biological environment in vivo,have recently garnered great attention because they often promote levels of cell differentiation and tissue organization not possible in conventional 2D culture systems. Here,we review the advances in researches of hepatic differentiation from stem cells in different 3D culture systems,focus on the materials used to make the 3D culture scaffolds.
