遗传
遺傳
유전
Hereditas
2015年
9期
873-884
,共12页
庞晓楠%弘笑%魏璇%陈喜文%刘佳%陈德富
龐曉楠%弘笑%魏璇%陳喜文%劉佳%陳德富
방효남%홍소%위선%진희문%류가%진덕부
乳铁蛋白%抑菌%抗病毒%免疫调节%重组表达系统
乳鐵蛋白%抑菌%抗病毒%免疫調節%重組錶達繫統
유철단백%억균%항병독%면역조절%중조표체계통
lactoferrin%antibacterial%antiviral%immunomodulatory%recombinant expression system
乳铁蛋白(Lactoferrin, Lf)是分子量大小约为80 kDa的铁离子结合糖蛋白,是转铁蛋白(Transferrin, Tf)家族的成员之一。其理化性质独特,具有抑菌、抗病毒、抗癌、免疫调节、调节铁离子的吸收等诸多生物学功能。获得高产且有生物活性的重组乳铁蛋白,并用于临床治疗,一直是研究热点。随着基因工程技术的发展,已获得多个可表达重组乳铁蛋白的表达系统。本文对乳铁蛋白的理化性质、生物学活性、临床研究以及目前的重组表达系统进行综述,以期为乳铁蛋白的临床应用提供参考。
乳鐵蛋白(Lactoferrin, Lf)是分子量大小約為80 kDa的鐵離子結閤糖蛋白,是轉鐵蛋白(Transferrin, Tf)傢族的成員之一。其理化性質獨特,具有抑菌、抗病毒、抗癌、免疫調節、調節鐵離子的吸收等諸多生物學功能。穫得高產且有生物活性的重組乳鐵蛋白,併用于臨床治療,一直是研究熱點。隨著基因工程技術的髮展,已穫得多箇可錶達重組乳鐵蛋白的錶達繫統。本文對乳鐵蛋白的理化性質、生物學活性、臨床研究以及目前的重組錶達繫統進行綜述,以期為乳鐵蛋白的臨床應用提供參攷。
유철단백(Lactoferrin, Lf)시분자량대소약위80 kDa적철리자결합당단백,시전철단백(Transferrin, Tf)가족적성원지일。기이화성질독특,구유억균、항병독、항암、면역조절、조절철리자적흡수등제다생물학공능。획득고산차유생물활성적중조유철단백,병용우림상치료,일직시연구열점。수착기인공정기술적발전,이획득다개가표체중조유철단백적표체계통。본문대유철단백적이화성질、생물학활성、림상연구이급목전적중조표체계통진행종술,이기위유철단백적림상응용제공삼고。
Lactoferrin is an iron-binding glycoprotein with a molecular weight of about 80 kDa that belongs to the transferrin family. Due to its unique physical and chemical properties, lactoferrin has a variety of biological functions in-cluding antibacterial, antiviral, anticancer, immunomodulatory activities and regulation of iron absorption. High-yield pro-duction of recombinant lactoferrin with biological activity and its application in clinical treatment have been a hot topic for long time. With the development of genetic engineering techniques, various expression systems have been developed to produce recombinant lactoferrin. In this review, we summarize physicochemical characteristics, biological activities, clinical studies and current recombinant expression systems of lactoferrin, in order to provide references for its clinical application.