国际眼科杂志
國際眼科雜誌
국제안과잡지
International Eye Science
2015年
10期
1691-1694
,共4页
曲古抑菌素A%成纤维细胞%转化生长因子-β1
麯古抑菌素A%成纖維細胞%轉化生長因子-β1
곡고억균소A%성섬유세포%전화생장인자-β1
trichostatin A%fibroblast%transforming growth factor -β1
目的：探讨组蛋白去乙酰化酶抑制剂( histone deacetylase inhibitor,HDACi )曲古抑菌素 A ( trichostatin A, TSA )对TGF-β1诱导人 Tenon 囊成纤维细胞( human Tenon fibroblasts, HTFs)增殖及玉型胶原纤维合成的影响。<br> 方法：首先,将不同浓度(200、400、600、800 nmol/L ) TSA作用HTFs共24h后,MTT法检测其对细胞活力的影响；然后,不同浓度(400、600nmol/L)TSA与5ng/mL TGF-β1混合作用于HTFs共24h,MTT法检测其对细胞活力的影响；最后,RT-PCR和Western-blot法分别检测不同浓度(400、600nmol/L ) TSA 与5ng/mL TGF-β1混合以及600 nmol/L TSA对HTFs玉型胶原纤维的mRNA及蛋白表达的影响。<br> 结果：MTT证实,与对照组相比,400nmol/L 及以上浓度TSA作用组, HTFs 活力显著下降( P<0.05)；两种浓度(400、600nmol/L)TSA均可减弱TGF-β1促HTFs增殖的作用( P<0.05)；RT-PCR和Western-blot证实两种浓度(400、600nmol/L)TSA对TGF-β1诱导上调的玉型胶原纤维在基因转录及蛋白表达水平有逆转作用。<br> 结论：TSA能够抑制TGF-β1诱导的HTFs增殖,并减弱玉型胶原纤维基因转录及蛋白表达水平。
目的：探討組蛋白去乙酰化酶抑製劑( histone deacetylase inhibitor,HDACi )麯古抑菌素 A ( trichostatin A, TSA )對TGF-β1誘導人 Tenon 囊成纖維細胞( human Tenon fibroblasts, HTFs)增殖及玉型膠原纖維閤成的影響。<br> 方法：首先,將不同濃度(200、400、600、800 nmol/L ) TSA作用HTFs共24h後,MTT法檢測其對細胞活力的影響；然後,不同濃度(400、600nmol/L)TSA與5ng/mL TGF-β1混閤作用于HTFs共24h,MTT法檢測其對細胞活力的影響；最後,RT-PCR和Western-blot法分彆檢測不同濃度(400、600nmol/L ) TSA 與5ng/mL TGF-β1混閤以及600 nmol/L TSA對HTFs玉型膠原纖維的mRNA及蛋白錶達的影響。<br> 結果：MTT證實,與對照組相比,400nmol/L 及以上濃度TSA作用組, HTFs 活力顯著下降( P<0.05)；兩種濃度(400、600nmol/L)TSA均可減弱TGF-β1促HTFs增殖的作用( P<0.05)；RT-PCR和Western-blot證實兩種濃度(400、600nmol/L)TSA對TGF-β1誘導上調的玉型膠原纖維在基因轉錄及蛋白錶達水平有逆轉作用。<br> 結論：TSA能夠抑製TGF-β1誘導的HTFs增殖,併減弱玉型膠原纖維基因轉錄及蛋白錶達水平。
목적：탐토조단백거을선화매억제제( histone deacetylase inhibitor,HDACi )곡고억균소 A ( trichostatin A, TSA )대TGF-β1유도인 Tenon 낭성섬유세포( human Tenon fibroblasts, HTFs)증식급옥형효원섬유합성적영향。<br> 방법：수선,장불동농도(200、400、600、800 nmol/L ) TSA작용HTFs공24h후,MTT법검측기대세포활력적영향；연후,불동농도(400、600nmol/L)TSA여5ng/mL TGF-β1혼합작용우HTFs공24h,MTT법검측기대세포활력적영향；최후,RT-PCR화Western-blot법분별검측불동농도(400、600nmol/L ) TSA 여5ng/mL TGF-β1혼합이급600 nmol/L TSA대HTFs옥형효원섬유적mRNA급단백표체적영향。<br> 결과：MTT증실,여대조조상비,400nmol/L 급이상농도TSA작용조, HTFs 활력현저하강( P<0.05)；량충농도(400、600nmol/L)TSA균가감약TGF-β1촉HTFs증식적작용( P<0.05)；RT-PCR화Western-blot증실량충농도(400、600nmol/L)TSA대TGF-β1유도상조적옥형효원섬유재기인전록급단백표체수평유역전작용。<br> 결론：TSA능구억제TGF-β1유도적HTFs증식,병감약옥형효원섬유기인전록급단백표체수평。
AIM:To investigate the effect of histone deacetylase inhibitor ( HDACi ) trichostatin A ( TSA ) on synthesis of collagen玉and proliferation of human Tenon fibroblasts ( HTFs ) induced by transforming growth factor- β1 ( TGF-β1 ) . <br> METHODS: Firstly, the effect of TSA at different concentrations (200, 400, 600 and 800nmol/L) on HTFs viability after 24h was detected using MTT proliferation assays. Then, the effect of TSA at 400nmol/L and 600nmol/L mixed with 5ng/mL TGF-β1 on HTFs viability after 24h were investigated using MTT proliferation assays. Furthermore, the mRNA and protein expression of collagen 玉 in HTFs after treatment with TSA at different concentrations ( 400, 600nmol/L ) mixed with 5ng/mL TGF-β1 as well as 600nmol/L TSA were detected by RT-PCR and Western-blot. <br> RESULTS:Compared with control group, the results of MTT showed that HTFs viability decreased significantly after treated with TSA at 400, 600 and 800nmol/L ( P<0.05). The HTFs proliferation induced by TGF-β1 could be attenuated by TSA at 400 and 600nmol/L (P<0. 05). The results of RT-PCR and Western-blot confirmed that TSA at 400 and 600nmol/L had reversal effect on up-regulated gene transcription and protein expression levels of collagen 玉 induced TGF-β1 . <br> CONCLUSION:TSA can inhibit the HTFs proliferation induced by TGF-β1 and attenuate gene transcription and protein expression of collagen 玉.
