遗传
遺傳
유전
Hereditas
2015年
9期
855-864
,共10页
周学%杜宜兰%金萍%马飞
週學%杜宜蘭%金萍%馬飛
주학%두의란%금평%마비
MicroRNAs(miRNAs)%癌症%靶基因%宿主基因%生物信息学
MicroRNAs(miRNAs)%癌癥%靶基因%宿主基因%生物信息學
MicroRNAs(miRNAs)%암증%파기인%숙주기인%생물신식학
microRNAs (miRNAs)%cancer%target gene%host gene%bioinformatics
MicroRNAs(miRNAs)是一类长度约为22nt的内源性非编码RNA，通过与靶基因转录本互补结合调控基因的表达。近年来，研究发现miRNA与癌症发生密切相关，miRNA可以直接充当癌基因或者抑癌基因而影响肿瘤的发生和生长。为更进一步揭示癌症相关 miRNA的特征及靶基因的功能，文章通过数据库搜索及文献检索，在人类基因组中发现了475个癌症相关miRNA，系统地比较了癌症相关miRNA与非癌症miRNA以及基因内和基因间区癌症相关 miRNA 在保守性、SNP 位点分布、癌谱及转录调控等特性。研究发现，癌症相关miRNA比非癌症miRNA保守性要强，发生SNP概率比较低，同时发现miRNA所涉及癌症数目与保守性成正相关。基因组定位分析发现，癌症相关 miRNA 比非癌症 miRNA 更倾向于成簇存在。进一步对宿主基因、癌症相关 miRNA 及作用的靶基因与癌症发生进行关联分析，发现一些非癌症 miRNA 的宿主基因倾向于被癌症miRNA作用。本研究结果为深入理解miRNA与癌症之间的关系，以及进一步为miRNA作为癌症诊断指示物提供理论依据。
MicroRNAs(miRNAs)是一類長度約為22nt的內源性非編碼RNA，通過與靶基因轉錄本互補結閤調控基因的錶達。近年來，研究髮現miRNA與癌癥髮生密切相關，miRNA可以直接充噹癌基因或者抑癌基因而影響腫瘤的髮生和生長。為更進一步揭示癌癥相關 miRNA的特徵及靶基因的功能，文章通過數據庫搜索及文獻檢索，在人類基因組中髮現瞭475箇癌癥相關miRNA，繫統地比較瞭癌癥相關miRNA與非癌癥miRNA以及基因內和基因間區癌癥相關 miRNA 在保守性、SNP 位點分佈、癌譜及轉錄調控等特性。研究髮現，癌癥相關miRNA比非癌癥miRNA保守性要彊，髮生SNP概率比較低，同時髮現miRNA所涉及癌癥數目與保守性成正相關。基因組定位分析髮現，癌癥相關 miRNA 比非癌癥 miRNA 更傾嚮于成簇存在。進一步對宿主基因、癌癥相關 miRNA 及作用的靶基因與癌癥髮生進行關聯分析，髮現一些非癌癥 miRNA 的宿主基因傾嚮于被癌癥miRNA作用。本研究結果為深入理解miRNA與癌癥之間的關繫，以及進一步為miRNA作為癌癥診斷指示物提供理論依據。
MicroRNAs(miRNAs)시일류장도약위22nt적내원성비편마RNA，통과여파기인전록본호보결합조공기인적표체。근년래，연구발현miRNA여암증발생밀절상관，miRNA가이직접충당암기인혹자억암기인이영향종류적발생화생장。위경진일보게시암증상관 miRNA적특정급파기인적공능，문장통과수거고수색급문헌검색，재인류기인조중발현료475개암증상관miRNA，계통지비교료암증상관miRNA여비암증miRNA이급기인내화기인간구암증상관 miRNA 재보수성、SNP 위점분포、암보급전록조공등특성。연구발현，암증상관miRNA비비암증miRNA보수성요강，발생SNP개솔비교저，동시발현miRNA소섭급암증수목여보수성성정상관。기인조정위분석발현，암증상관 miRNA 비비암증 miRNA 경경향우성족존재。진일보대숙주기인、암증상관 miRNA 급작용적파기인여암증발생진행관련분석，발현일사비암증 miRNA 적숙주기인경향우피암증miRNA작용。본연구결과위심입리해miRNA여암증지간적관계，이급진일보위miRNA작위암증진단지시물제공이론의거。
expression by targeting complementary transcripts. Recent studies have found that miRNAs are closely related to tumorigenesis and can act as oncogenes or tumor suppressor genes to influence the occurrence and development of tumor. To further reveal characteristics of cancer-related miRNAs and the functions of miRNA targets, we obtained 475 miRNAs involved in cancer through database searching and information retrieval. We systematically analyzed and compared the features including conservation, SNP distribution, cancer spectrum width, and transcriptional regulation between cancer and non-cancer related miRNAs as well as between intergenic and intragenic miRNAs. Our results showed that cancer-related miRNAs have higher conservation and lower SNP frequency compared to non-cancer-related miRNAs, and the cancer spectrum of one miRNA is positively correlated with its conservation. Genome analysis showed that cancer-related miRNAs tend to present as clusters compared with non-cancer-related miRNAs. Further association analysis between cancer progression and host genes, cancer-related miRNAs or target genes found that the host genes of some non-cancer related miRNAs tend to be targeted by cancer-related miRNAs. This study provides theoretical basis for further understanding the relationship between miRNA and cancer progression as well as the miRNA-based cancer diagnosis.
