CAJ | 학술논문

【目的】采用纽约大学脊髓重物坠落伤仪制作轻、中、重型损伤的稳定的大鼠脊髓挫伤模型。【方法】成年雌性 SD 大鼠,咬除 T10~11棘突及相应椎板,暴露脊髓硬脊膜,采用纽约大学脊髓重物坠落伤仪从12.5 cm(A组)、25 cm(B 组)、和50 cm(C 组)三种不同高度10 g 对暴露脊髓撞击,造成脊髓挫伤,对照组(D 组)仅暴露硬脊膜不撞击,选择不同的时间点对各组实验大鼠进行行为学评分和病理检测。【结果】D 组麻醉恢复后能站立行走,脊髓结构正常；A 组术后4周运动功能基本恢复,运动功能评分维持在17.34±1.00,病理切片可见脊髓结构清晰,胶质细胞增生；B 组术后4周运动功能评分维持在7.32±1.12,病理切片可见腔隙形成,胶质细胞大量增生,少量变形神经元细胞；C 组术后4周运动功能评分维持在4.87±0.31,残留脊髓组织比 B 组少,脊髓结构紊乱,严重变形,坏死空腔约占脊髓80%以上。四组运动功能评分比较均有统计学意义(P <0.05)。【结论】采用纽约大学脊髓重物坠落伤仪制作的轻、中、重型大鼠脊髓挫伤模型,稳定可靠,重复性良好,可用于研究脊髓损伤机制、病理生理改变和筛选各种临床治疗方案等。
【목적】채용뉴약대학척수중물추락상의제작경、중、중형손상적은정적대서척수좌상모형。【방법】성년자성 SD 대서,교제 T10~11극돌급상응추판,폭로척수경척막,채용뉴약대학척수중물추락상의종12.5 cm(A조)、25 cm(B 조)、화50 cm(C 조)삼충불동고도10 g 대폭로척수당격,조성척수좌상,대조조(D 조)부폭로경척막불당격,선택불동적시간점대각조실험대서진행행위학평분화병리검측。【결과】D 조마취회복후능참립행주,척수결구정상；A 조술후4주운동공능기본회복,운동공능평분유지재17.34±1.00,병리절편가견척수결구청석,효질세포증생；B 조술후4주운동공능평분유지재7.32±1.12,병리절편가견강극형성,효질세포대량증생,소량변형신경원세포；C 조술후4주운동공능평분유지재4.87±0.31,잔류척수조직비 B 조소,척수결구문란,엄중변형,배사공강약점척수80%이상。사조운동공능평분비교균유통계학의의(P <0.05)。【결론】채용뉴약대학척수중물추락상의제작적경、중、중형대서척수좌상모형,은정가고,중복성량호,가용우연구척수손상궤제、병리생리개변화사선각충림상치료방안등。
[Objective]To established mild,moderate and severe models of spinal cord injury with a MASCIS im-pactor.[Methods]In female adult Sprague-Dawley rats,T10 spinous process and the corresponding vertebral lamina were removed and spinal cord endorhachis was exposed.Three levels of injury were inflicted to rat spinal cord by dropping a 10-g rod 12.5,25.0 or 50.0 mm on to exposed spinal cord.The control group underwent T10~1 1 laminectomies alone.The be-havioral scores and pathological alterations were recorded at different timepoints.[Results]In control group,the rats could walk after reviving and the micro-structure of spinal cord was normal.Group A (12.5 mm),1 month after opera-tion,movement function was almost restored,the score of Basso,Beattie & Bresnahan (BBB)was 17.34±1.00,micro-structure of spinal cord was distinct,glial cell hyperplasia;group B (25 mm),1 month after operation,the score of BBB was 7.32±1.12,lacunar formation,glial cell hyperplasia and several variant neurons were present in micro-structure of spinal cord;group C (50 mm),1 month after operation,the score of BBB was 4.87±0.31,residue spinal cord tissues were less abundant than group B.The spinal cord structure was disorderly and seriously deformed.The necrotic cavity ac-counted for about 80% of spinal cord.The grading differences were statistically significant between groups A,B and C and normal control (P <0.05).[Conclusion]The models of three levels of mild,moderate and severe injury made by a MASCIS impactor are stable,reliable and reproducible.they can used for elucidating the mechanism of spinal cord injury, pathophysiological changes and different treatments.