CAJ | 학술논문

目的探讨细胞支架蛋白palladin在胰腺癌中的表达及其临床病理意义.方法收集56例胰腺癌(PDAC)患者肿瘤组织及癌旁正常胰腺(NP)组织,另取10例慢性胰腺炎(CP)患者CP组织.应用Western blot法和免疫组织化学法检测palladin蛋白在3组胰腺组织中的表达,并分析其与胰腺癌临床病理因素间的关系.结果 Western blot测定结果显示,PDAC、CP和NP组织中palladin蛋白表达水平分别为0.93±0.07、0.41±0.07、0.20±0.06,PDAC组织palladin蛋白表达高于其他两组(P＜0.05),CP组织中palladin蛋白表达高于NP组织(P＜0.05).免疫组织化学结果显示,palladin蛋白表达于活化的肌成纤维细胞中,PDAC组织中palladin蛋白高表达率为79％(44/56),高于NP组织的2/10和CP组织的4/10(P＜ 0.05),且胰腺癌中palladin蛋白表达与肿瘤分化程度、淋巴结转移、临床TNM分期有关(P＜0.05),与患者性别、年龄、肿瘤部位、有无远处转移无关(P＞0.05).结论细胞支架蛋白palladin在胰腺癌中高表达,且表达于肿瘤微环境内活化的肌成纤维细胞中,可能参与胰腺癌的侵袭和转移过程.
목적 탐토세포지가단백palladin재이선암중적표체급기림상병리의의.방법 수집56례이선암(PDAC)환자종류조직급암방정상이선(NP)조직,령취10례만성이선염(CP)환자CP조직.응용Western blot법화면역조직화학법검측palladin단백재3조이선조직중적표체,병분석기여이선암림상병리인소간적관계.결과 Western blot측정결과현시,PDAC、CP화NP조직중palladin단백표체수평분별위0.93±0.07、0.41±0.07、0.20±0.06,PDAC조직palladin단백표체고우기타량조(P＜0.05),CP조직중palladin단백표체고우NP조직(P＜0.05).면역조직화학결과현시,palladin단백표체우활화적기성섬유세포중,PDAC조직중palladin단백고표체솔위79％(44/56),고우NP조직적2/10화CP조직적4/10(P＜ 0.05),차이선암중palladin단백표체여종류분화정도、림파결전이、림상TNM분기유관(P＜0.05),여환자성별、년령、종류부위、유무원처전이무관(P＞0.05).결론 세포지가단백palladin재이선암중고표체,차표체우종류미배경내활화적기성섬유세포중,가능삼여이선암적침습화전이과정.
Objective To investigate the expression of scaffold protein palladin in pancreatic ductal adenocarcinoma (PDAC) tissues and to discuss its clinicopathological significance.Methods 56 samples of PDAC and corresponding adjacent normal pancreas (NP) tissues were collected.Another 10 samples of chronic pancreatitis (CP) tissues were collected.Western blot analysis and immunohistochemistry assay were performed to detect the expression of protein palladin.The correlation of palladin expression with clinicopathological factors of PDAC was analyzed.Results Western blot analysis revealed that the expression of palladin in PDAC,CP and NP tissues were respectively 0.93±0.07,0.41±0.07 and 0.20±0.06,and the expression of palladin was significantly increased in PDAC tissues compared with NP and CP tissues (P ＜ 0.05),and its expression was significantly increased in CP tissues compared with NP tissues (P ＜ 0.05).Immunohistochemical staining showed that palladin was mainly expressed in activated myofibroblasts in PDAC tissues.The rate of palladin expression was 79 ％ (44/56),which was higher than that in NP tissues (2/10) and CP tissues (4/10),and its expression was found to be correlated with the degree of tumor differentiation,lymph node metastasis and clinical TNM classification (P ＜ 0.05),and it had no correlation with patient' s sex,age,tumor location and distant metastasis (P ＞ 0.05).Conclusions Scaffold protein palladin is highly expressed in PDAC tissues,and it is expressed in the activated myofibroblasts within tumor microenvironment.Scaffold protein palladin may be involved in the invasion and metastasis of PDAC.