肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
Cancer Research and Clinic
2015年
8期
505-509,514
,共6页
刘瑞廷%王国荣%邱健%王小强%阎立昆%李小军%刘昌
劉瑞廷%王國榮%邱健%王小彊%閻立昆%李小軍%劉昌
류서정%왕국영%구건%왕소강%염립곤%리소군%류창
结直肠肿瘤%DNA结合蛋白A%免疫组织化学%反转录聚合酶链反应%印迹法,蛋白质
結直腸腫瘤%DNA結閤蛋白A%免疫組織化學%反轉錄聚閤酶鏈反應%印跡法,蛋白質
결직장종류%DNA결합단백A%면역조직화학%반전록취합매련반응%인적법,단백질
Colorectal neoplasms%DNA binding protein A%Immunohistochemistry%Reverse transcriptase polymerase chain reaction%Blotting,western
目的 研究DNA结合蛋白A(dbpA)基因在结直肠癌组织和细胞中的表达及其临床意义.方法 采用免疫组织化学方法检测60例结直肠癌患者癌组织及癌旁正常结直肠黏膜组织中dbpA蛋白的表达,采用免疫荧光及反转录PCR法检测结直肠黏膜上皮细胞系FHC、人结直肠腺癌细胞系SW480、RKO、SW620、DLD-1、HT-29、SW1463及患者癌组织、癌旁组织中dbpA mRNA表达,Western blot法检测结直肠癌组织及细胞中蛋白的表达变化.结果 在正常结直肠组织中dbpA蛋白和mRNA无表达或低表达,在结直肠癌组织中dbpA表达明显增高.结直肠癌中dbpA mRNA阳性表达率升高,分别为80.0％(48/60)、10.0％(6/60),dbpA蛋白阳性表达率升高,分别为83.3％(50/60)、10.0％(6/60),差异均有统计学意义(P＜0.01).在FHC细胞中dbpA无表达,在6株结直肠癌细胞中,dbpA不同程度高表达(P＜0.05).dbpA高表达与结直肠癌浸润深度、淋巴结转移、组织学分型及脉管侵犯相关(P＜0.05),dbpA高表达组预后较差(P＜0.05).结论 dbpA的升高可能在结直肠癌的发生发展中起重要作用,可能是结直肠癌的一个预后指标.
目的 研究DNA結閤蛋白A(dbpA)基因在結直腸癌組織和細胞中的錶達及其臨床意義.方法 採用免疫組織化學方法檢測60例結直腸癌患者癌組織及癌徬正常結直腸黏膜組織中dbpA蛋白的錶達,採用免疫熒光及反轉錄PCR法檢測結直腸黏膜上皮細胞繫FHC、人結直腸腺癌細胞繫SW480、RKO、SW620、DLD-1、HT-29、SW1463及患者癌組織、癌徬組織中dbpA mRNA錶達,Western blot法檢測結直腸癌組織及細胞中蛋白的錶達變化.結果 在正常結直腸組織中dbpA蛋白和mRNA無錶達或低錶達,在結直腸癌組織中dbpA錶達明顯增高.結直腸癌中dbpA mRNA暘性錶達率升高,分彆為80.0％(48/60)、10.0％(6/60),dbpA蛋白暘性錶達率升高,分彆為83.3％(50/60)、10.0％(6/60),差異均有統計學意義(P＜0.01).在FHC細胞中dbpA無錶達,在6株結直腸癌細胞中,dbpA不同程度高錶達(P＜0.05).dbpA高錶達與結直腸癌浸潤深度、淋巴結轉移、組織學分型及脈管侵犯相關(P＜0.05),dbpA高錶達組預後較差(P＜0.05).結論 dbpA的升高可能在結直腸癌的髮生髮展中起重要作用,可能是結直腸癌的一箇預後指標.
목적 연구DNA결합단백A(dbpA)기인재결직장암조직화세포중적표체급기림상의의.방법 채용면역조직화학방법검측60례결직장암환자암조직급암방정상결직장점막조직중dbpA단백적표체,채용면역형광급반전록PCR법검측결직장점막상피세포계FHC、인결직장선암세포계SW480、RKO、SW620、DLD-1、HT-29、SW1463급환자암조직、암방조직중dbpA mRNA표체,Western blot법검측결직장암조직급세포중단백적표체변화.결과 재정상결직장조직중dbpA단백화mRNA무표체혹저표체,재결직장암조직중dbpA표체명현증고.결직장암중dbpA mRNA양성표체솔승고,분별위80.0％(48/60)、10.0％(6/60),dbpA단백양성표체솔승고,분별위83.3％(50/60)、10.0％(6/60),차이균유통계학의의(P＜0.01).재FHC세포중dbpA무표체,재6주결직장암세포중,dbpA불동정도고표체(P＜0.05).dbpA고표체여결직장암침윤심도、림파결전이、조직학분형급맥관침범상관(P＜0.05),dbpA고표체조예후교차(P＜0.05).결론 dbpA적승고가능재결직장암적발생발전중기중요작용,가능시결직장암적일개예후지표.
Objective To investigate the expression of DNA binding protein A (dbpA) in patients with colorectal carcinoma of different stages and its significance.Methods Expression of dbpA protein and mRNA in specimens of normal tissues and colorectal cancer were detected by immunohistochemistry,expression of dbpA mRNA and protein of colorectal cancer cell lines SW480,RKO,SW620,DLD-1,HT-29,SW1463 and tissues were detected by immunohistochemistry,RT-PCR and Western blot.Results There was no positive staining dbpA protein and mRNA in normal colorectal tissues.However,dbpA was expressed in epithelial cells of colorectal mucosa [dbpA mRNA:80.0 ％ (48/60),10.0 ％ (6/60);dbpA protein:83.3 ％ (50/60),10.0 ％ (6/60),P ＜ 0.01].And there was no expression in normal colorectal cell,but its expression was high in 6 colorectal cancer cells (P ＜ 0.05).The high expression of dbpA was correlation with the infiltration depth,lymph node metastasis and type of histology (P ＜ 0.05),and had effect in prognosis of colorectal cancer (P ＜ 0.05).Conclusion Elevated dbpA may be related to the pathogenesis and development of colorectal carcinoma,and dbpA may be a prognostic factor of colorectal carcinoma.
