CAJ | 학술논문

传统酒曲的运用是酿造名优白酒并形成独特风味的基础之一.随着微生物分类学技术的日益进步,揭示传统大曲中微生物多样性结构,对探明风味形成机理、优化生产工艺具有重要意义.简介了选择培养、生化鉴定、基因文库、DGGE等的基础性和适用性;探讨了质谱、定量PCR、高通量测序等的高效性及可行性.
전통주곡적운용시양조명우백주병형성독특풍미적기출지일.수착미생물분류학기술적일익진보,게시전통대곡중미생물다양성결구,대탐명풍미형성궤리、우화생산공예구유중요의의.간개료선택배양、생화감정、기인문고、DGGE등적기출성화괄용성;탐토료질보、정량PCR、고통량측서등적고효성급가행성.
The traditional Daqu is the key factor to produce famous Baijiu(liquor) with special flavor. With the increasing improvement in mi-crobial taxonomic techniques, the exploration of microbial diversity structure of the traditional Daqu is of great significance in the research on the formation mechanism of Baijiu(liquor) flavor, and the optimization of Daqu production techniques. In this paper, the applicability of bio-chemical identification, gene library, DGGE etc. was introduced briefly. The efficiency and the suitability of mass spectrum, real-time PCR, high throughout sequencing etc. were also elaborated.