安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
Acta Universitatis Medicinalis Anhui
2015年
9期
1247-1251
,共5页
熊宇%黄涛%吴奎%刘治%周杭城
熊宇%黃濤%吳奎%劉治%週杭城
웅우%황도%오규%류치%주항성
高脂高糖%丙酸睾酮%前列腺指数%前列腺增生%胰岛素生长因子1%胰岛素生长因子1受体
高脂高糖%丙痠睪酮%前列腺指數%前列腺增生%胰島素生長因子1%胰島素生長因子1受體
고지고당%병산고동%전렬선지수%전렬선증생%이도소생장인자1%이도소생장인자1수체
high-fat and high-sugar diet%testosterone%prostate index%benign prostatic hyperplasia%IGF-1%IGF-1 R
目的 研究不同饮食和丙酸睾酮对SD大鼠前列腺组织的改变及胰岛素生长因子( IGF)-1、IGF-1R 的表达强度. 方法 采用高脂高糖饲料饮食及去势后皮下注射丙酸睾酮建造前列腺增生模型,40 d后处死大鼠;取出前列腺组织,称重并HE染色观察前列腺增生情况;用免疫组化法检测前列腺组织中IGF-1、IGF-1R的表达强度. 结果 高脂高糖饮食组前列腺组织增生,IGF-1、IGF-1R的表达强度均高于普通饮食组(P<0. 05);去势+低剂量丙酸睾酮皮下注射组前列腺组织明显增生,IGF-1、IGF-1R的表达强度明显增加(P<0. 05). 结论 高脂高糖饮食及低剂量丙酸睾酮均可促进大鼠前列腺组织的增生,IGF-1、IGF-1R与前列腺增生的发展呈正相关性.
目的 研究不同飲食和丙痠睪酮對SD大鼠前列腺組織的改變及胰島素生長因子( IGF)-1、IGF-1R 的錶達彊度. 方法 採用高脂高糖飼料飲食及去勢後皮下註射丙痠睪酮建造前列腺增生模型,40 d後處死大鼠;取齣前列腺組織,稱重併HE染色觀察前列腺增生情況;用免疫組化法檢測前列腺組織中IGF-1、IGF-1R的錶達彊度. 結果 高脂高糖飲食組前列腺組織增生,IGF-1、IGF-1R的錶達彊度均高于普通飲食組(P<0. 05);去勢+低劑量丙痠睪酮皮下註射組前列腺組織明顯增生,IGF-1、IGF-1R的錶達彊度明顯增加(P<0. 05). 結論 高脂高糖飲食及低劑量丙痠睪酮均可促進大鼠前列腺組織的增生,IGF-1、IGF-1R與前列腺增生的髮展呈正相關性.
목적 연구불동음식화병산고동대SD대서전렬선조직적개변급이도소생장인자( IGF)-1、IGF-1R 적표체강도. 방법 채용고지고당사료음식급거세후피하주사병산고동건조전렬선증생모형,40 d후처사대서;취출전렬선조직,칭중병HE염색관찰전렬선증생정황;용면역조화법검측전렬선조직중IGF-1、IGF-1R적표체강도. 결과 고지고당음식조전렬선조직증생,IGF-1、IGF-1R적표체강도균고우보통음식조(P<0. 05);거세+저제량병산고동피하주사조전렬선조직명현증생,IGF-1、IGF-1R적표체강도명현증가(P<0. 05). 결론 고지고당음식급저제량병산고동균가촉진대서전렬선조직적증생,IGF-1、IGF-1R여전렬선증생적발전정정상관성.
Objective To study different diet and testosterone propionate on SD rat prostatic tissue changes and in-sulin-like growth factor ( insulin-like growth factor, IGF)-1, IGF-1R expression of strength. Methods High fat and high-sugar diet after subcutaneous injection of testosterone propionate castrated prostatic hyperplasia model con-struction. All were killed 40 days later, removed the prostate tissue, weighed and HE staining prostatic hyperplasia cases were detected by immunohistochemistry in prostate tissue IGF-1, IGF-1R expression intensity. Results High fat and high sugar diet group prostatic hyperplasia and IGF-1 , IGF-1 R expression intensity was higher than normal diet group ( P<0. 05 );high fat and high-sugar diet and low-dose subcutaneous injection of testosterone propionate group obviously prostate hyperplasia, IGF-1, IGF-1R expression intensity were significantly increased (P<0. 05). Conclusion High-fat and high-sugar diet low dose of exogenous testosterone supplements can promote the prolifer-ation of rat prostate tissue, IGF-1, IGF-1R and the development of benign prostatic hyperplasia are positively corre-lated.