应用化工
應用化工
응용화공
Applied Chemical Industry
2015年
9期
1751-1753
,共3页
易雪静%周石柔%邓兰青%徐中海
易雪靜%週石柔%鄧蘭青%徐中海
역설정%주석유%산란청%서중해
水芹%氨基酸%反相高效液相色谱
水芹%氨基痠%反相高效液相色譜
수근%안기산%반상고효액상색보
oenanthe javanica%amino acids%reversed phase high performance liquid chromatography
利用反相高效液相色谱法对东洞庭湖区野生水芹茎及叶中氨基酸成分进行分析,水芹茎及叶经过烘干后用6 mol/L盐酸消解,利用邻苯二甲醛进行衍生,荧光法测定。结果表明,水芹茎及叶所含15种氨基酸总量分别为1.882 g/100 g和6.939 g/100 g,这一结果为评价野生水芹的营养价值提供了一定的依据。该方法快速、准确、结果可靠,适用于水芹中氨基酸的检测。
利用反相高效液相色譜法對東洞庭湖區野生水芹莖及葉中氨基痠成分進行分析,水芹莖及葉經過烘榦後用6 mol/L鹽痠消解,利用鄰苯二甲醛進行衍生,熒光法測定。結果錶明,水芹莖及葉所含15種氨基痠總量分彆為1.882 g/100 g和6.939 g/100 g,這一結果為評價野生水芹的營養價值提供瞭一定的依據。該方法快速、準確、結果可靠,適用于水芹中氨基痠的檢測。
이용반상고효액상색보법대동동정호구야생수근경급협중안기산성분진행분석,수근경급협경과홍간후용6 mol/L염산소해,이용린분이갑철진행연생,형광법측정。결과표명,수근경급협소함15충안기산총량분별위1.882 g/100 g화6.939 g/100 g,저일결과위평개야생수근적영양개치제공료일정적의거。해방법쾌속、준학、결과가고,괄용우수근중안기산적검측。
By using RP-HPLC, the amino acids contents of wild oenanthe javanica stems and leaves in Dongting lake area was analyzed. Wild oenanthe javanica stems and leaves are dried first,by counteraction with 6 mol/L hydrochloric acid,and then are derived using o-phthaldialdehyde( OPA) for determination by fluorophotometry. The result showed that 15 kinds of amino acid contents of oenanthe javanica stems and leaves were 6. 939 g/100 g and 1. 882 g/100 g,respectively. The results provide some basis for the evaluation of the nutritional value of wild oenanthe javanica. The method is rapid,accurate reliable,and suitable for determination of amino acids from oenanthe javanica.