CAJ | 학술논문

目的研究兴奋下丘脑腹外侧视前区( VLPO)对结节乳头体核( TMN) c-Fos表达的影响及其受体途径. 方法免疫组织化学方法检测VLPO代谢型谷氨酸受体5 ( mGluR5 )和TMNγ-氨基丁酸A受体β1亚型(GABAARβ1)的表达;采用脑立体定位技术,观察微量注射谷氨酸( L-Glu)兴奋VL-PO后对TMN c-Fos表达变化的影响. 结果 mGluR5于VL-PO存在阳性表达,同时GABAA Rβ1于TMN存在阳性表达;与对照组相比,VLPO内微量注射L-Glu后,大鼠的TMN区域c-Fos表达量明显减少,差异有统计学意义( P <0. 01 ).结论存在于VLPO和TMN的mGluR及GABAA R介导VL-PO-TMN的通路调节,VLPO可通过GABAA R抑制TMN神经元活动.
목적 연구흥강하구뇌복외측시전구( VLPO)대결절유두체핵( TMN) c-Fos표체적영향급기수체도경. 방법 면역조직화학방법검측VLPO대사형곡안산수체5 ( mGluR5 )화TMNγ-안기정산A수체β1아형(GABAARβ1)적표체;채용뇌입체정위기술,관찰미량주사곡안산( L-Glu)흥강VL-PO후대TMN c-Fos표체변화적영향. 결과 mGluR5우VL-PO존재양성표체,동시GABAA Rβ1우TMN존재양성표체;여대조조상비,VLPO내미량주사L-Glu후,대서적TMN구역c-Fos표체량명현감소,차이유통계학의의( P <0. 01 ).결론 존재우VLPO화TMN적mGluR급GABAA R개도VL-PO-TMN적통로조절,VLPO가통과GABAA R억제TMN신경원활동.
Objective To investigate the effect of c-Fos expression in tuberomammillary nucleus ( TMN) after ex-citing ventrolateral preoptic ( VLPO) and its receptor passway. Methods The expressions of mGluR5 in VLPO and GABAA Rβ1 in TMN were determined by immunostaining. L-Glutamate ( L-Glu) was microinjected into VLPO with stereotaxic technique to observe the changes of c-Fos expression in TMN. Results Positive protein of mGluR5 could be identified by Anti-mGluR5 in VLPO and also positive protein of GABAAR was identified in TMN by Anti-GABAARβ1. c-Fos expression in TMN reduced after microinjecting L-Glu in VLPO compared with the control group. The difference between them was significant (P<0. 01). Conclusion The metabotropic glutamate recep-tor in VLPO and GABA receptor in TMN mediate the regulation of VLPO-TMN passway. VLPO inhibits the TMN neuronal activity via GABAAR.