CAJ | 학술논문

为了研究 ABA 对北方白菜型冬油菜抗寒性的影响，本试验以白菜型冬油菜陇油8号为材料，分别于冬油菜冬前三、五、六、七叶期用10 mg／L 的脱落酸（ABA）溶液喷施叶片，以各个时期喷蒸馏水为对照，分析其对越冬率、产量及生理生化指标的影响。结果表明，外源 ABA 喷施能够显著提高白菜型冬油菜的越冬率和产量，提高保护性酶活性及渗透调节物等生理生化物质含量，且这种作用主要发生在受到低温（－5℃）胁迫以后，ABA 处理的越冬率和产量分别较 CK 增加14．3％，8．0％；受低温胁迫后的 SOD、POD、CAT 酶的活性分别较 CK 提高39．8％，20．8％，31．0％；SP、SS、Pro 含量较 CK 增加104．5％，120．1％，77．7％，且随喷施时期总体呈先升高后降低的趋势，六叶期喷施效果最佳。同时 ABA 处理能增强根系活力，降低 MDA 含量。因此，在北方寒旱区，在白菜型冬油菜冬前六叶期喷施一定浓度的 ABA 能提高冬油菜的抗寒性。
위료연구 ABA 대북방백채형동유채항한성적영향，본시험이백채형동유채롱유8호위재료，분별우동유채동전삼、오、륙、칠협기용10 mg／L 적탈락산（ABA）용액분시협편，이각개시기분증류수위대조，분석기대월동솔、산량급생리생화지표적영향。결과표명，외원 ABA 분시능구현저제고백채형동유채적월동솔화산량，제고보호성매활성급삼투조절물등생리생화물질함량，차저충작용주요발생재수도저온（－5℃）협박이후，ABA 처리적월동솔화산량분별교 CK 증가14．3％，8．0％；수저온협박후적 SOD、POD、CAT 매적활성분별교 CK 제고39．8％，20．8％，31．0％；SP、SS、Pro 함량교 CK 증가104．5％，120．1％，77．7％，차수분시시기총체정선승고후강저적추세，륙협기분시효과최가。동시 ABA 처리능증강근계활력，강저 MDA 함량。인차，재북방한한구，재백채형동유채동전륙협기분시일정농도적 ABA 능제고동유채적항한성。
The aim of this study was to determine the effects of exogenous abscisic acid (ABA)on the cold re-sistance of Brassica rapa (winter rape,cultivar Longyou 8)under low temperature stress.Leaves of Longyou 8 were sprayed with 10 mg/L ABA when the plants were at the three-,five-,six-,and seven-leaf stages before winter.Plants at the same stages were sprayed with distilled water as the control.The overwintering rate, yield and physiological and biochemical indexes of Longyou 8 were determined after a cold treatment (-5℃). The plants sprayed with ABA showed significantly increased overwintering rates and yields,higher activities of antioxidant enzymes,and increased contents of physiological and biochemical substances that function as os-motic regulators.The effect was extremely significant after low temperature stress,when the overwintering rate and yield of ABA-treated plants were 14.3% and 8.0% higher than their respective values in the control plants.The activities of superoxide dismutase,peroxidase,and catalase were 39.8%,20.8%,31.0% higher, respectively,in ABA-treated plants than in control plants after the low temperature treatment.The contents of soluble protein,soluble saccharides,and free proline were 104.5%,120.1%,and 77.7% higher,respectively, in the ABA-treated plants than in control plants after the low temperature treatment.The best stage for ABA application was the six-leaf stage.Application of ABA also increased root activity and decreased the contents of malondialdehyde,a marker of lipid peroxidation.Together,these results show that spraying with ABA can im-prove the cold resistance of winter rape in northern China.