CAJ | 학술논문

目的：评价X E‐5000全自动血液细胞分析仪检测外周血有核红细胞（N RBC ）的主要性能特点，并探讨其临床应用价值。方法对XE‐5000全自动血液细胞分析仪的主要性能进行检测，并选取137份经分析仪DIFF通道检测提示NRBC阳性的患者血液标本，采用分析仪NRBC通道检测NRBC计数和百分比，采用显微镜分类检测NRBC百分比，采用SPSS18．0统计软件进行统计分析。结果高、中和低值标本的N RBC计数及N RBC百分比的批内精密度分别为2．10％、3．26％、11．62％和3．79％、5．80％、13．33％，携带污染率NRBC计数为0．51％，NRBC百分比为0．26％，在（0～18）×109／L范围内XE‐5000全自动血液细胞分析仪对NRBC的检测结果呈良好的线性：Y＝1．0486X＋0．1896（r＝0．9991）。XE‐5000全自动血液细胞分析仪与显微镜分类计数法的 N RBC百分比检测结果比较差异无统计学意义（ P＝0．716），呈良好的相关性：Y ＝1．1502 X＋0．6261（ r＝0．9670）。结论 XE‐5000全自动血液细胞分析仪可替代传统的显微镜分类计数方法应用于临床。
목적：평개X E‐5000전자동혈액세포분석의검측외주혈유핵홍세포（N RBC ）적주요성능특점，병탐토기림상응용개치。방법대XE‐5000전자동혈액세포분석의적주요성능진행검측，병선취137빈경분석의DIFF통도검측제시NRBC양성적환자혈액표본，채용분석의NRBC통도검측NRBC계수화백분비，채용현미경분류검측NRBC백분비，채용SPSS18．0통계연건진행통계분석。결과고、중화저치표본적N RBC계수급N RBC백분비적비내정밀도분별위2．10％、3．26％、11．62％화3．79％、5．80％、13．33％，휴대오염솔NRBC계수위0．51％，NRBC백분비위0．26％，재（0～18）×109／L범위내XE‐5000전자동혈액세포분석의대NRBC적검측결과정량호적선성：Y＝1．0486X＋0．1896（r＝0．9991）。XE‐5000전자동혈액세포분석의여현미경분류계수법적 N RBC백분비검측결과비교차이무통계학의의（ P＝0．716），정량호적상관성：Y ＝1．1502 X＋0．6261（ r＝0．9670）。결론 XE‐5000전자동혈액세포분석의가체대전통적현미경분류계수방법응용우림상。
Objective To evaluate the performance of XE‐5000 automated blood cell analyser for detecting nucleated red blood cell (NRBC) in peripheral blood and investigate its clinical application value .Methods The intra‐assay imprecision ,carryover rate , and linear range of the analyser were evaluated .The absolute NRBC count and percentage of NRBC of 137 blood specimens (NRBC‐positive according to the DIFF channel of the analyser) were determined in the NRBC channel of the analyser ,and the percentage of NRBC of these blood specimens were determined by using microscope method as well .Differences between the two methods were analysed by using SPSS18 .0 statistic software .Results The intra‐assay imprecision of the analyser for detecting absolute NRBC count in specimens with high ,moderate ,and low Q‐flag values were 2 .10% ,3 .26% and 11 .62% ,respectively ,and the imprecision for detecting percentage of NRBC were 3 .79% ,5 .80% and 13 .33% ,respectively .The carryover rates of the analyser for detecting absolute NRBC count and percentage of NRBC were 0 .51% and 0 .26% ,respectively .At the range of (0~18)× 109/L ,the absolute NRBC count detected by using the analyser showed good linearity :Y=1 .048 6X+0 .189 6(r=0 .999 1) .There were no statistically significant differences between the analyser and microscope for detecting percentage of NRBC(P=0 .716) ,and showed good corre‐lation:Y=1 .150 2X+0 .626 1(r=0 .967 0) .Conclusion The XE‐5000 automated blood cell analyser could completely replace the traditional microscope for clinically classifying and counting NRBCs .