草业学报
草業學報
초업학보
Acta Prataculturae Sinica
2015年
9期
154-162
,共9页
刘芳%田忠平%蔡英杰%张雨%周蕴薇
劉芳%田忠平%蔡英傑%張雨%週蘊薇
류방%전충평%채영걸%장우%주온미
细叶百合%鳞茎%休眠%淀粉粒%花芽分化
細葉百閤%鱗莖%休眠%澱粉粒%花芽分化
세협백합%린경%휴면%정분립%화아분화
Lilium pumilum%bulbs%dormancy%starch grains%flower bud differentiation
以细叶百合为试材,通过低温(5℃)解除鳞茎休眠,研究了休眠解除过程中鳞茎细胞的淀粉粒的变化及细叶百合花芽分化的变化过程。通过石蜡切片和实体显微结构观察,结果表明,低温冷藏期间,顶芽生长锥的高度和宽度逐渐增加,细叶百合花芽分化主要分为4个时期,0~48 d 为小花原基分化期,60 d 为外轮花被原基分化期,72 d为内轮花被原基分化期,84 d 为雄蕊和雌蕊原基分化期;鳞片及顶芽细胞内淀粉粒数量随着冷藏时间的延长逐渐减少。冷藏0~24 d 内,鳞茎细胞没有进行有丝分裂,冷藏36 d 以后细胞分裂数量逐渐增加,冷藏84 d 分裂期细胞数量增加到2.6个。
以細葉百閤為試材,通過低溫(5℃)解除鱗莖休眠,研究瞭休眠解除過程中鱗莖細胞的澱粉粒的變化及細葉百閤花芽分化的變化過程。通過石蠟切片和實體顯微結構觀察,結果錶明,低溫冷藏期間,頂芽生長錐的高度和寬度逐漸增加,細葉百閤花芽分化主要分為4箇時期,0~48 d 為小花原基分化期,60 d 為外輪花被原基分化期,72 d為內輪花被原基分化期,84 d 為雄蕊和雌蕊原基分化期;鱗片及頂芽細胞內澱粉粒數量隨著冷藏時間的延長逐漸減少。冷藏0~24 d 內,鱗莖細胞沒有進行有絲分裂,冷藏36 d 以後細胞分裂數量逐漸增加,冷藏84 d 分裂期細胞數量增加到2.6箇。
이세협백합위시재,통과저온(5℃)해제린경휴면,연구료휴면해제과정중린경세포적정분립적변화급세협백합화아분화적변화과정。통과석사절편화실체현미결구관찰,결과표명,저온랭장기간,정아생장추적고도화관도축점증가,세협백합화아분화주요분위4개시기,0~48 d 위소화원기분화기,60 d 위외륜화피원기분화기,72 d위내륜화피원기분화기,84 d 위웅예화자예원기분화기;린편급정아세포내정분립수량수착랭장시간적연장축점감소。랭장0~24 d 내,린경세포몰유진행유사분렬,랭장36 d 이후세포분렬수량축점증가,랭장84 d 분렬기세포수량증가도2.6개。
Changes in starch grains and flower bud differentiation of lily (Lilium pumilum )were studied during the breaking of dormancy at 5℃.Observations of paraffin sections under a microscope revealed that the height of the growth cone and the width of apical buds increased gradually during cold storage.The flower bud differ-entiation process in lily could be divided into four stages:the floral primordium differentiation stage from 0 to 48 days,the outer perianth primordium differentiation stage at 60 days,the inner perianth primordium differ-entiation stage at 72 days,and the stamen and pistil primordium differentiation stage at 84 days.The number of starch grains in apical bud cells gradually decreased as the duration of the cold temperature treatment extend-ed.There were almost no mitotic cells in the plant tissues from 0 to 24 days of cold storage.The number of mitotic cells increased gradually after 36 days in cold storage,reaching 2.6 at 84 days of cold storage.