草业学报
草業學報
초업학보
Acta Prataculturae Sinica
2015年
9期
146-153
,共8页
钴胁迫%紫花苜蓿%种子萌发%幼苗生长%抗氧化酶%渗透调节%活性氧
鈷脅迫%紫花苜蓿%種子萌髮%幼苗生長%抗氧化酶%滲透調節%活性氧
고협박%자화목숙%충자맹발%유묘생장%항양화매%삼투조절%활성양
cobalt stress%Medicago sativa%seed germination%seedling growth%antioxidant enzyme%osmotic adjustment%active oxygen
以“甘农3号”紫花苜蓿为试验材料,研究不同浓度 Co2+(0,0.25,0.50,1.00,2.50,5.00 mmol/L CoCl2)胁迫对紫花苜蓿种子萌发及幼苗生理生化特性的影响。结果表明:Co 胁迫对紫花苜蓿种子的萌发及幼苗的生长有明显的抑制作用,随着 Co 胁迫浓度的增大,种子发芽势、发芽率、发芽指数、活力指数及幼苗的胚芽长、胚根长、根系活力和干重均显著降低,而且 Co 胁迫对发芽势的抑制作用大于发芽率,对胚根生长的抑制作用大于胚芽;低浓度Co 胁迫(0.25和0.50 mmol/L)下,苜蓿幼苗叶片中可溶性蛋白、可溶性糖含量和蛋白水解酶、超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、愈创木酚过氧化物酶(GPX)、过氧化氢酶(CAT)活性与 CK 均无显著差异,而游离脯氨酸含量显著升高;随着 Co 胁迫浓度的升高,可溶性蛋白、可溶性糖、游离脯氨酸含量及蛋白水解酶、SOD、APX、GPX、CAT 活性均显著下降,超氧阴离子自由基(O2·-)产生速率、羟自由基(OH ·)浓度、H2 O2及丙二醛(MDA)含量均显著增加。说明高浓度 Co 胁迫使得苜蓿幼苗抗氧化系统活性下降,活性氧清除能力降低,膜脂过氧化程度加剧,从而抑制了紫花苜蓿种子的萌发及幼苗的生长。
以“甘農3號”紫花苜蓿為試驗材料,研究不同濃度 Co2+(0,0.25,0.50,1.00,2.50,5.00 mmol/L CoCl2)脅迫對紫花苜蓿種子萌髮及幼苗生理生化特性的影響。結果錶明:Co 脅迫對紫花苜蓿種子的萌髮及幼苗的生長有明顯的抑製作用,隨著 Co 脅迫濃度的增大,種子髮芽勢、髮芽率、髮芽指數、活力指數及幼苗的胚芽長、胚根長、根繫活力和榦重均顯著降低,而且 Co 脅迫對髮芽勢的抑製作用大于髮芽率,對胚根生長的抑製作用大于胚芽;低濃度Co 脅迫(0.25和0.50 mmol/L)下,苜蓿幼苗葉片中可溶性蛋白、可溶性糖含量和蛋白水解酶、超氧化物歧化酶(SOD)、抗壞血痠過氧化物酶(APX)、愈創木酚過氧化物酶(GPX)、過氧化氫酶(CAT)活性與 CK 均無顯著差異,而遊離脯氨痠含量顯著升高;隨著 Co 脅迫濃度的升高,可溶性蛋白、可溶性糖、遊離脯氨痠含量及蛋白水解酶、SOD、APX、GPX、CAT 活性均顯著下降,超氧陰離子自由基(O2·-)產生速率、羥自由基(OH ·)濃度、H2 O2及丙二醛(MDA)含量均顯著增加。說明高濃度 Co 脅迫使得苜蓿幼苗抗氧化繫統活性下降,活性氧清除能力降低,膜脂過氧化程度加劇,從而抑製瞭紫花苜蓿種子的萌髮及幼苗的生長。
이“감농3호”자화목숙위시험재료,연구불동농도 Co2+(0,0.25,0.50,1.00,2.50,5.00 mmol/L CoCl2)협박대자화목숙충자맹발급유묘생리생화특성적영향。결과표명:Co 협박대자화목숙충자적맹발급유묘적생장유명현적억제작용,수착 Co 협박농도적증대,충자발아세、발아솔、발아지수、활력지수급유묘적배아장、배근장、근계활력화간중균현저강저,이차 Co 협박대발아세적억제작용대우발아솔,대배근생장적억제작용대우배아;저농도Co 협박(0.25화0.50 mmol/L)하,목숙유묘협편중가용성단백、가용성당함량화단백수해매、초양화물기화매(SOD)、항배혈산과양화물매(APX)、유창목분과양화물매(GPX)、과양화경매(CAT)활성여 CK 균무현저차이,이유리포안산함량현저승고;수착 Co 협박농도적승고,가용성단백、가용성당、유리포안산함량급단백수해매、SOD、APX、GPX、CAT 활성균현저하강,초양음리자자유기(O2·-)산생속솔、간자유기(OH ·)농도、H2 O2급병이철(MDA)함량균현저증가。설명고농도 Co 협박사득목숙유묘항양화계통활성하강,활성양청제능력강저,막지과양화정도가극,종이억제료자화목숙충자적맹발급유묘적생장。
The aim of this study was to investigate the mechanism of cobalt (Co)stress damage on Medicago sativa as experiment materials,The effects of different concentrations (0,0.25,0.50,1.00,2.50,5.00 mmol/L CoCl2 )of Co on M.sativa (cv.Gannong No.3)seed germination and seedling physiological and bio-chemical characteristics were assessed.The results indicate that Co stress significantly inhibited seed germina-tion and seedling growth.Seed germination potential,germination rate,germination index,vigor index,seed-ling sprout length,radicle length,root activity and dry weight were significantly reduced with increasing cobalt concentration.The inhibitory effects of Co stress on germination potential was higher than that of cobalt stress on germination rate,and the inhibitory effects of Co stress on radical growth was higher than that on the plu-mule.Co significantly reduced the soluble protein content,soluble sugar content,proteolytic enzyme activity,superoxide dismutase (SOD),ascorbate peroxidase (APX),guaiacol peroxidase (GPX)and catalase (CAT)in seedling leaves.However free proline content increased dramatically at lower Co concentrations but declined at higher concentrations.At higher Co concentrations,O2 ·- (superoxide anion radical),OH · (hydroxyl radical), H2 O2 and malondialdehyde increased significantly.This illustrates that under high Co stress,the seedling an-tioxidant system activity and active oxygen scavenging ability of M.sativa decreased,inhibiting M.sativa seed germination and seedling growth.