CAJ | 학술논문

以二茂铁、亮氨酸、缬氨酸和赖氨酸为原料,苯并三唑-1-四甲基六氟磷酸酯( HBTU)及1-羟基苯并三唑( HOBt)为缩合剂,采用液相合成法,合成了Fc-Lys-Leu-Val-OCH3(简称Fc-KLV,其中Fc：ferrocenoyl,二茂铁).用柱层析分离纯化得到目标化合物,并对目标化合物进行紫外和核磁表征以确证其结构. Fc-KLV的循环伏安扫描结果表明,在0.4~0.8 V范围内出现了一对可逆的氧化还原峰,氧化峰和还原峰电位分别为624 mV和546 mV,峰电位之差ΔEp为78 mV,峰电流密度之比Ipa/Ipc =1.12,扩散系数D=1.782×10-6 cm2/s,表明相应的电化学过程主要受扩散控制.
이이무철、량안산、힐안산화뢰안산위원료,분병삼서-1-사갑기륙불린산지( HBTU)급1-간기분병삼서( HOBt)위축합제,채용액상합성법,합성료Fc-Lys-Leu-Val-OCH3(간칭Fc-KLV,기중Fc：ferrocenoyl,이무철).용주층석분리순화득도목표화합물,병대목표화합물진행자외화핵자표정이학증기결구. Fc-KLV적순배복안소묘결과표명,재0.4~0.8 V범위내출현료일대가역적양화환원봉,양화봉화환원봉전위분별위624 mV화546 mV,봉전위지차ΔEp위78 mV,봉전류밀도지비Ipa/Ipc =1.12,확산계수D=1.782×10-6 cm2/s,표명상응적전화학과정주요수확산공제.
Electrochemical peptide Fc-Lys-Leu-Val-OCH3 ( Fc-KLV, Fc:ferrocenoyl ) was synthesized from ferrocene,Leu,Val and Lys by solution phase peptide synthesis method u-sing O-( benzotriazol-yl)-N,N,N’,N’-tetramethyluronium ( HBTU)/1-hydroxybenzotrizole ( HOBt) as coupling reagents. The crude Fc-peptide was purified by column chromatogra-phy,and then was characterized by UV and NMR. The formal redox potential,Epa and Epc at 624 mV and 546 mV,respectively,and Ipa/Ipcat 1. 12 were characteristic for the Fc-pep-tide undergoing a one-electron redox process. These results suggested that the electrochemi-cal process is mainly controlled by the diffusion.