CAJ | 학술논문

目的：研究树脂单体甲基丙烯酸β-羟乙酯（HEMA）对人牙髓细胞（hDPCs）增殖和迁移的影响。方法：组织块法体外原代培养获得 hDPCs，以含不同浓度 HEMA 的 DMEM培养基与第3～5代 hDPCs 共同培养，用 MTT 实验检测 HEMA 作用于hDPCs 24、48、72 h 的增殖情况，Transwell 法观察细胞的迁移能力。结果：HEMA 在体外一定程度上抑制了 hDPCs 的增殖（与对照组比较，P ＜0．05），并呈时间浓度依赖性，24 h 组的细胞活力值均显著高于48 h 和72 h 组（P ＜0．05）。HEMA 减少了 hD-PCs 的迁移数（P ＜0．05）。结论：HEMA 体外能抑制人牙髓细胞的增殖和迁移。
목적：연구수지단체갑기병희산β-간을지（HEMA）대인아수세포（hDPCs）증식화천이적영향。방법：조직괴법체외원대배양획득 hDPCs，이함불동농도 HEMA 적 DMEM배양기여제3～5대 hDPCs 공동배양，용 MTT 실험검측 HEMA 작용우hDPCs 24、48、72 h 적증식정황，Transwell 법관찰세포적천이능력。결과：HEMA 재체외일정정도상억제료 hDPCs 적증식（여대조조비교，P ＜0．05），병정시간농도의뢰성，24 h 조적세포활력치균현저고우48 h 화72 h 조（P ＜0．05）。HEMA 감소료 hD-PCs 적천이수（P ＜0．05）。결론：HEMA 체외능억제인아수세포적증식화천이。
Objective:To assess the effects of HEMA on the proliferation and migration of human dental pulp cells(hDPCs).Meth-ods:hDPCs were obtained using tissue explant culture technique in vitro,and then cells of the 3rd -5th passages were treated by differ-ent concentrations of HEMA for 24,48 and 72 h respectively.Cell proliferation was examined by MTT assay.Cell migration was ob-served by Transwell method.Results:The proliferation ability of hDPCs decreased when exposed to HEMA in both time and concentra-tion dependent manner(vs control,P <0.05).Cell proliferation at 24 h expossure was statistically higher than that at 48 h and 72 h(P<0.05).The migration of hDPCs was significantly reduced in HEMA groups at different concentrations(vs control,P <0.05).Con-clusion:HEMA inhibits the proliferation and migration of human dental pulp cells in vitro.