CAJ | 학술논문

目的：研究DNase I对金黄色葡萄球菌生物膜形成的影响。方法采用分光光度计法检测金黄色葡萄球菌的生长曲线，菌落计数法分析金黄色葡萄球菌的粘附能力，96孔板结晶紫染色法检测金黄色葡萄球菌生物膜的形成。结果不同浓度DNase I对金黄色葡萄球菌的生长无影响，然而能显著抑制细菌生物膜的形成，并呈浓度依赖性。此外，DNase I能抑制不同生长期金黄色葡萄球菌的粘附性。与抗菌药物和DNase I单独应用相比，DNase I和抗菌药物联合应用更能显著抑制和分解金黄色葡萄球菌成熟生物膜。结论 DNase I可以有效抑制金黄色葡萄球菌生物膜的形成，并能增强抗菌药物对金黄色葡萄球菌生物膜的抑制作用。
목적：연구DNase I대금황색포도구균생물막형성적영향。방법채용분광광도계법검측금황색포도구균적생장곡선，균락계수법분석금황색포도구균적점부능력，96공판결정자염색법검측금황색포도구균생물막적형성。결과불동농도DNase I대금황색포도구균적생장무영향，연이능현저억제세균생물막적형성，병정농도의뢰성。차외，DNase I능억제불동생장기금황색포도구균적점부성。여항균약물화DNase I단독응용상비，DNase I화항균약물연합응용경능현저억제화분해금황색포도구균성숙생물막。결론 DNase I가이유효억제금황색포도구균생물막적형성，병능증강항균약물대금황색포도구균생물막적억제작용。
Objective To study the effect of DNase I on biofilm formation of Staphylococcus aureus. Methods The growth curve of S. aureus was detected using a spectrophotometer. The adhesion of S. aureus was analyzed using flat colony counting method, and the biofilm formation was assayed using the 96-well crystal violet staining method. Results Exposure to different concentrations of DNase I did not obviously affect the growth of S. aureus but significantly inhibit the formation of bacterial biofilms in a dose-dependent manner. DNase I inhibited the adhesion of S. aureus at different growth stages. When combined with antibiotics, DNase I resulted in a significant decrease in the established biofilm biomass compared to antibiotics or DNase I used alone. Conclusion DNase I can effectively inhibit biofilm formation of S. aureus and enhance the inhibitory effect of antibiotics against S. aureus biofilms.