CAJ | 학술논문

目的：研究大鼠腮腺主导管结扎诱导腺体萎缩过程中腺泡细胞的变化规律。方法：通过对大鼠腮腺主导管结扎，建立腮腺组织萎缩模型，分别于结扎术后0、1、3、5、7、10、14、21和30 d 获取腮腺组织标本，苏木精－伊红（HE）和阿新蓝－过碘酸雪夫（AB-PAS）染色观察腺体的组织学变化，免疫组织化学法检测半胱氨酸天冬氨酸蛋白水解酶3（caspase3）、增殖细胞核抗原（Ki-67）、钙调节蛋白（calponin）和淀粉酶（amylase）的表达变化。结果：组织学观察见导管结扎组大鼠腮腺大部分腺泡细胞萎缩、消失，酶原颗粒消失，仅在腺小叶的边缘残存少量腺泡细胞。Caspase3在结扎7 d 组达峰值；Ki-67在结扎3 d组达峰值；各实验组间 caspase3及 Ki-67表达水平差异均有统计学意义（P ＜0．05）。结论：大鼠腮腺主导管结扎至30 d 时，大部分腺泡细胞萎缩、凋亡，分泌功能丧失，但在腺小叶的边缘仍存在少量腺泡细胞。
목적：연구대서시선주도관결찰유도선체위축과정중선포세포적변화규률。방법：통과대대서시선주도관결찰，건립시선조직위축모형，분별우결찰술후0、1、3、5、7、10、14、21화30 d 획취시선조직표본，소목정－이홍（HE）화아신람－과전산설부（AB-PAS）염색관찰선체적조직학변화，면역조직화학법검측반광안산천동안산단백수해매3（caspase3）、증식세포핵항원（Ki-67）、개조절단백（calponin）화정분매（amylase）적표체변화。결과：조직학관찰견도관결찰조대서시선대부분선포세포위축、소실，매원과립소실，부재선소협적변연잔존소량선포세포。Caspase3재결찰7 d 조체봉치；Ki-67재결찰3 d조체봉치；각실험조간 caspase3급 Ki-67표체수평차이균유통계학의의（P ＜0．05）。결론：대서시선주도관결찰지30 d 시，대부분선포세포위축、조망，분비공능상실，단재선소협적변연잉존재소량선포세포。
Objective:To investigate the changes of acinar cells during atrophy of rat duct-ligated parotid gland.Methods:The ex-cretory duct of parotid gland was doubly ligated with metal-clip unilaterally near the hilum,and the animals were sacrificed at 0,1 , 3,5,7,1 0,1 4,21 or 30 days after ligation respectively.The evolving glands were examined with HE and AB-PAS staining tech-nique and immunohistochemistry for the observation of caspase3,Ki-67,calponin and amylase expression.Results:30-days after ligation,the majority of acinar cells were disappeared;only residual acinar cells at the peripheral region of lobules were identified by HE and AB-PAS staining accompanying decreasing zymogen granules.During the atrophy of parotid glands,the caspase3-positive cells identified by immunohistochemistry were rarely observed at 0 d,but the cell number increased in the following days.There were occasional Ki-67 positive cells in the 0 d group,but after 3-days of the ligation Ki-67 positive cells reached a peak.The difference of the caspase3-positive cell number and the Ki-67 positive cells were statistically significant among the groups(P <0.05).Conclu-sion:During atrophy of the parotid gland,most acinar cells apoptosed but there are still some residual acinar cells at the perip-heral region of lobules 30 days after duct-ligation.