CAJ | 학술논문

目的：建立黄连种植土壤中酚酸类物质的分析方法，探索其累积与消减规律。方法：采用FeCl3-K3[Fe(CN)6]比色法测定黄连种植土壤中总酚酸的含量，采用HPLC法测定黄连种植基地土壤中阿魏酸的含量。结果：测得黄连种植基地土壤中总酚酸含量约0.545-2.026 mg·100g-1在范围内，阿魏酸含量在0.139-0.652μ g·100g-1范围内。结论：黄连种植土壤中酚酸含量变化规律明显，栽培过程中随黄连生长年限的增加，连地土壤中总酚酸与阿魏酸的含量均表现为增加，采收黄连后的休地过程中，土壤中总酚酸与阿魏酸的含量均呈现递减趋势，该变化趋势可为黄连连作障碍研究机制与解决方法研究提供了参考。
목적：건립황련충식토양중분산류물질적분석방법，탐색기루적여소감규률。방법：채용FeCl3-K3[Fe(CN)6]비색법측정황련충식토양중총분산적함량，채용HPLC법측정황련충식기지토양중아위산적함량。결과：측득황련충식기지토양중총분산함량약0.545-2.026 mg·100g-1재범위내，아위산함량재0.139-0.652μ g·100g-1범위내。결론：황련충식토양중분산함량변화규률명현，재배과정중수황련생장년한적증가，련지토양중총분산여아위산적함량균표현위증가，채수황련후적휴지과정중，토양중총분산여아위산적함량균정현체감추세，해변화추세가위황련련작장애연구궤제여해결방법연구제공료삼고。
This study was aimed to establish a method to determine the content of phenolic acids in the soil of cultivation base ofCoptis chinensis, in order to study the accumulation and decrease law of phenolic acids. The content of total phenolic acid was determined by ferric chloride-ferricyanatum calcium colorimetric method. Thecontent of ferulic acid in Coptis chinensis was determined by HPLC. The results showed that the contents of total phenolic acid and ferulic acid in the soil of cultivation base of Coptis chinensis were in the range from 0.545-0.026 mg·g-1 and 0.139 to 0.652 μg·g-1, respectively. It was concluded that the variation of phenolic acids in the soil of cultivation base ofCoptis chinensis was obvious. With the increase of growth age of Coptis chinensis, the contents of total phenolic acid and ferulic acid in the soil of cultivation base of Coptis chinensis were increased in the cultivation period. With the increase of fallow age, the contents of total phenolic acid and ferulic acid in the soil of cultivation base ofCoptis chinensis showed decrease tendency in the fallow period of Coptis chinensis. The variation tendency of phenolic acids contents in the soil of cultivation base ofCoptis chinensis can be referred to in the study of the continuous cropping obstacle of Coptis chinensis.