CAJ | 학술논문

目的：研究银翘散复方挥发油稳定性的影响因素。方法采用毛细管气相色谱-质谱联用(GC-MS)测定连续提取复方挥发油的化学成分，研究挥发油提取的重复性，并以复方挥发油吸光度为其稳定性的评价指标，考察光照、温度、溶液pH值和金属离子对银翘散复方挥发油吸光度的影响。结果 GC-MS分析结果表眀，间隔15 d的2次提取的银翘散挥发油化学成分种类相同。稳定性试验结果表明，复方挥发油低温(4℃)避光保存其吸光度变化缓慢，在光照或常温条件下其吸光度的稳定性较差，强酸强碱性条件下其吸光度变化较大、挥发油不稳定。金属离子铜离子和铁离子与复方挥发油中的化学成分发生反应，紫外吸收光谱中形成新的吸收峰。结论复方挥发油应低温避光存放，同时避免与酸碱试剂、铜离子或铁离子接触以保证其稳定性。本研究为银翘散复方挥发油的保存条件和制剂条件提供了参考。
목적：연구은교산복방휘발유은정성적영향인소。방법채용모세관기상색보-질보련용(GC-MS)측정련속제취복방휘발유적화학성분，연구휘발유제취적중복성，병이복방휘발유흡광도위기은정성적평개지표，고찰광조、온도、용액pH치화금속리자대은교산복방휘발유흡광도적영향。결과 GC-MS분석결과표명，간격15 d적2차제취적은교산휘발유화학성분충류상동。은정성시험결과표명，복방휘발유저온(4℃)피광보존기흡광도변화완만，재광조혹상온조건하기흡광도적은정성교차，강산강감성조건하기흡광도변화교대、휘발유불은정。금속리자동리자화철리자여복방휘발유중적화학성분발생반응，자외흡수광보중형성신적흡수봉。결론복방휘발유응저온피광존방，동시피면여산감시제、동리자혹철리자접촉이보증기은정성。본연구위은교산복방휘발유적보존조건화제제조건제공료삼고。
Objective To investigate the factors affecting the stability of the volatile oil extracted fromYinqiaosan Decoction.Methods The main chemical compositions and the extraction repetitiveness of the compound volatile oil were determined by GC-MS, and the stability of multiple extracted volatile oil was studied. Absorbance of the compound volatile oil was used as the evaluation index, and the factors affecting the stability of the of the compound volatile oil were investigated, such as illumination, temperatures and pH values of volatile oil solution and metal ions.Results The results of the GC-MS chromatograph indicated that the main chemical compositions of the compound volatile oil extracted fromYinqiaosan Decoction twice were the same. The results of the stability of the volatile oil showed that the preservation temperature and illumination affected the stability of the volatile oil to a certain extent. The absorbance values of the compound volatile oil changed slowly when it was stored at a relatively low temperature (4℃) and shielded from light, and it was less stable when stored in normal temperature and under illumination. Meanwhile, the absorbance of the compound volatile oil changed quickly in acid or alkaline solutions and was in instability. The metal ions, such as Cu2+ and Fe3+, have chemical reactions with the compositions of the compound volatile oil and there was a big change in the UV-Vis spectrum of the compound volatile oils.Conclusion The compound volatile oil should be stored at a relatively low temperature (4℃) and shielded from light. At the same time, it should be stored avoiding acids, alkaline and the metal ions, such as Cu2+ and Fe3+, to guarantee its stability. This study provides a reference for the preservation conditions and the preparation conditions of the compound volatile oil extracted fromYinqiaosan Decoction.