中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
Chinese Journal of Information on Traditional Chinese Medicine
2015年
10期
77-80
,共4页
田七痛经胶囊%高效液相色谱法%人参皂苷Rg1%人参皂苷Rb1%三七皂苷R1
田七痛經膠囊%高效液相色譜法%人參皂苷Rg1%人參皂苷Rb1%三七皂苷R1
전칠통경효낭%고효액상색보법%인삼조감Rg1%인삼조감Rb1%삼칠조감R1
Tianqi Tongjing Capsule%HPLC%Ginsengnoside Rg1%Ginsengnoside Rb1%Notoginsenoside R1
目的：建立田七痛经胶囊的含量测定方法。方法采用安捷伦ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),流动相A为乙腈,流动相B为水,梯度洗脱(0～20 min,19%→21%A；20～50 min,21%A→36%A),检测波长203 nm,流速1.0 mg/mL,柱温25℃,测定田七痛经胶囊中人参皂苷Rg1、Rb1和三七皂苷R1的含量。结果人参皂苷Rg1、Rb1和三七皂苷R1的线性范围分别为0.4424～3.9816μg(r2＝1.0000)、0.5248～3.6736μg(r2＝0.9994)和0.2032～1.016μg (r2＝0.9992),平均回收率分别为99.49%(RSD＝2.44%)、99.02%(RSD＝2.45%)和99.98%(RSD＝2.14%)。结论本研究所建立的方法分离效果好、简便、快捷,重复性好,可有效控制田七痛经胶囊的质量。
目的：建立田七痛經膠囊的含量測定方法。方法採用安捷倫ZORBAX SB-C18色譜柱(4.6 mm×250 mm,5μm),流動相A為乙腈,流動相B為水,梯度洗脫(0～20 min,19%→21%A；20～50 min,21%A→36%A),檢測波長203 nm,流速1.0 mg/mL,柱溫25℃,測定田七痛經膠囊中人參皂苷Rg1、Rb1和三七皂苷R1的含量。結果人參皂苷Rg1、Rb1和三七皂苷R1的線性範圍分彆為0.4424～3.9816μg(r2＝1.0000)、0.5248～3.6736μg(r2＝0.9994)和0.2032～1.016μg (r2＝0.9992),平均迴收率分彆為99.49%(RSD＝2.44%)、99.02%(RSD＝2.45%)和99.98%(RSD＝2.14%)。結論本研究所建立的方法分離效果好、簡便、快捷,重複性好,可有效控製田七痛經膠囊的質量。
목적：건립전칠통경효낭적함량측정방법。방법채용안첩륜ZORBAX SB-C18색보주(4.6 mm×250 mm,5μm),류동상A위을정,류동상B위수,제도세탈(0～20 min,19%→21%A；20～50 min,21%A→36%A),검측파장203 nm,류속1.0 mg/mL,주온25℃,측정전칠통경효낭중인삼조감Rg1、Rb1화삼칠조감R1적함량。결과인삼조감Rg1、Rb1화삼칠조감R1적선성범위분별위0.4424～3.9816μg(r2＝1.0000)、0.5248～3.6736μg(r2＝0.9994)화0.2032～1.016μg (r2＝0.9992),평균회수솔분별위99.49%(RSD＝2.44%)、99.02%(RSD＝2.45%)화99.98%(RSD＝2.14%)。결론본연구소건립적방법분리효과호、간편、쾌첩,중복성호,가유효공제전칠통경효낭적질량。
Objective To establish an HPLC method for content determination of Ginsengnoside Rg1, Ginsengnoside Rb1 and Notoginsenoside R1 inTianqi Tongjing Capsule.Methods An Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5μm) was used. The mobile phase was composed of acetonitrile (A) and water (B) with gradient elution (0-20 min, 19%→21%A;20-50min, 21%→36%A) at a flow rate of 1.0 mg/mL;the wavelength of detector was 203 nm;the temperature of the column was 25℃.Results The calibration curves of Ginsengnoside Rg1, Ginsengnoside Rb1 and Notoginsenoside R1 showed good linearity within the range of 0.442 4-3.981 6μg (r2=1.000 0), 0.524 8-3.673 6μg (r2=0.999 4) and 0.203 2-1.016μg (r2=0.999 2), respectively. The average recoveries (n=9) were 99.49%, 99.02% and 99.98%, and RSD were 2.44%, 2.45% and 2.14%, respectively.Conclusion The method is simple, reliable, rapid and with good repeatability, and can effectively control the quality ofTianqi Tongjing Capsule.
