CAJ | 학술논문

目的：建立同时测定竹节参中人参皂苷Rg1、Re、Rb1含量的高效液相色谱方法。方法用70%乙醇超声提取竹节参药材,采用Agilent Poroshell 120 C18色谱柱(4.6 mm×100 mm,2.7μm),流动相为乙腈-0.1%磷酸,梯度洗脱,流速1.0 mL/min,检测波长203 nm,柱温30℃,进样量10μL。结果在该色谱条件下,人参皂苷Rg1、Re、Rb1达到基线分离,线性关系良好,加样回收率分别为99.5%、103.0%、100.5%。结论该方法操作简便,重复性好,分析周期短,能准确测定竹节参中人参皂苷Rg1、Re、Rb1的含量,可为竹节参的质量控制提供参考依据。
목적：건립동시측정죽절삼중인삼조감Rg1、Re、Rb1함량적고효액상색보방법。방법용70%을순초성제취죽절삼약재,채용Agilent Poroshell 120 C18색보주(4.6 mm×100 mm,2.7μm),류동상위을정-0.1%린산,제도세탈,류속1.0 mL/min,검측파장203 nm,주온30℃,진양량10μL。결과재해색보조건하,인삼조감Rg1、Re、Rb1체도기선분리,선성관계량호,가양회수솔분별위99.5%、103.0%、100.5%。결론해방법조작간편,중복성호,분석주기단,능준학측정죽절삼중인삼조감Rg1、Re、Rb1적함량,가위죽절삼적질량공제제공삼고의거。
Objective To develop an HPLC method for simultaneous content determination of ginsenoside Rg1, Re and Rb1 in Panacis Japonici Rhizoma.Methods Chromatographic separation was carried out by using an Agilent Poroshell 120 C18 column (4.6 mm × 100 mm, 2.7μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid with gradient elution at a flowrate of 1.0 mL/min, and the injection volume was 10μL. The detection wavelength and column temperature were 203 nm and 30℃ respectively.Results Ginsenoside Rg1, Re and Rb1 had the baseline separation and were in good linear range. The recovery rates were 99.5%, 103.0% and 100.5% respectively.Conclusion The approach is simple, accurate, with good repeatability and short analysis period, which can determine the contents of ginsenoside Rg1, Re and Rb1 correctly and provide references for quality control of Panacis Japonici Rhizoma.