CAJ | 학술논문

目的：观察左归降糖解郁方对糖尿病并发抑郁症大鼠海马谷氨酸、N-甲基-D-天冬氨酸受体(NMDA receptor,NR)2A及NR2B表达的影响,探讨其对糖尿病并发抑郁症大鼠海马损伤的保护机制。方法建立糖尿病并发抑郁症大鼠模型,随机分为模型组、阳性药组及左归降糖解郁方高、中、低剂量组,以正常大鼠为正常组,每组16只,各给药组给予相应药物灌胃,连续28 d。采用Open-field实验评价大鼠行为变化,ELISA检测海马谷氨酸含量,免疫荧光法检测海马NR2A、NR2B表达。结果与正常组比较,模型组大鼠自主活动次数明显减少(P＜0.01),海马谷氨酸含量明显升高(P＜0.01),NR2A、NR2B表达明显升高(P＜0.01)；与模型组比较,阳性药组和左归降糖解郁方高剂量组大鼠自主活动次数明显增加(P＜0.01),海马谷氨酸含量明显下降(P＜0.01), NR2A、NR2B表达降低(P＜0.05)。结论左归降糖解郁方可明显改善糖尿病并发抑郁症大鼠的抑郁行为,其药效作用可能与调控大鼠海马谷氨酸含量及NR2A、NR2B表达有关。
목적：관찰좌귀강당해욱방대당뇨병병발억욱증대서해마곡안산、N-갑기-D-천동안산수체(NMDA receptor,NR)2A급NR2B표체적영향,탐토기대당뇨병병발억욱증대서해마손상적보호궤제。방법건립당뇨병병발억욱증대서모형,수궤분위모형조、양성약조급좌귀강당해욱방고、중、저제량조,이정상대서위정상조,매조16지,각급약조급여상응약물관위,련속28 d。채용Open-field실험평개대서행위변화,ELISA검측해마곡안산함량,면역형광법검측해마NR2A、NR2B표체。결과여정상조비교,모형조대서자주활동차수명현감소(P＜0.01),해마곡안산함량명현승고(P＜0.01),NR2A、NR2B표체명현승고(P＜0.01)；여모형조비교,양성약조화좌귀강당해욱방고제량조대서자주활동차수명현증가(P＜0.01),해마곡안산함량명현하강(P＜0.01), NR2A、NR2B표체강저(P＜0.05)。결론좌귀강당해욱방가명현개선당뇨병병발억욱증대서적억욱행위,기약효작용가능여조공대서해마곡안산함량급NR2A、NR2B표체유관。
ObjectiveTo observe the effect ofZuogui Jiangtang Jieyu Formula on the expressions of glutamate, NR2A and NR2B in hippocampus of diabetic rats with depression;To explore the mechanism of protective effect. Methods Diabetes with depression rat models were established and then were randomly divided into the model group, positive medicine group, high-, medium- and low-doseZuogui Jiangtang Jieyu Formula groups. Normal rats acted as normal group, 16 rats per group. After 28 days of administration, Open-field test was used to detect the behavior of the rats;glutamate content of hippocampus was detected by ELISA;the expressions of NR2A and NR2B in rat hippocampus were detected by immunofluorescence.Results Compared with normal group, automatic activity times of rats in model group decreased significantly (P<0.01);both glutamate content (P<0.01) and expressions of NR2A, NR2B (P<0.01) increased significantly. Compared with the model group, automatic activity times of rats in positive medicine group and high-doseZuogui Jiangtang Jieyu Formula group significantly increased (P<0.01);glutamate content dropped (P<0.01);expressions of NR2A and NR2B decreased (P<0.05).ConclusionZuogui Jiangtang Jieyu Formula can improve depressive behavior of diabetic rats with depression, which may be related to the regulation of glutamate content and expressions of NR2A and NR2B in hippocampus.