CAJ | 학술논문

以预先合成的半乳糖化壳聚糖—氟尿嘧啶偶合物( GC-FUA)为原料,采用离子交联法制备出了GC-FUA纳米给药体系,并结合单因素分析法筛选出其最佳制备条件为GC-FUA浓度2.0 mg/mL,TPP浓度1.0 mg/mL,pH值4.5,GC-FUA/TPP质量比12∶1.应用SEM、UV等表征该纳米给药体系多呈球形,大小较均一,且具有缓释性能；采用MTT 法观察5-Fu、物理包封5-Fu GC纳米粒子、GC-FUA纳米粒子对HepG2细胞的增殖抑制作用.结果表明三者对HepG2细胞的增殖具有明显抑制作用,且呈剂量依赖性, GC-FUA纳米粒子作用较5-Fu和物理包封5-Fu GC纳米粒子明显增强.
이예선합성적반유당화각취당—불뇨밀정우합물( GC-FUA)위원료,채용리자교련법제비출료GC-FUA납미급약체계,병결합단인소분석법사선출기최가제비조건위GC-FUA농도2.0 mg/mL,TPP농도1.0 mg/mL,pH치4.5,GC-FUA/TPP질량비12∶1.응용SEM、UV등표정해납미급약체계다정구형,대소교균일,차구유완석성능；채용MTT 법관찰5-Fu、물리포봉5-Fu GC납미입자、GC-FUA납미입자대HepG2세포적증식억제작용.결과표명삼자대HepG2세포적증식구유명현억제작용,차정제량의뢰성, GC-FUA납미입자작용교5-Fu화물리포봉5-Fu GC납미입자명현증강.
In this experiment,N-galactosylated-chitosan-5-fluorouracil acetic acid conjugate ( GC- FUA ) which we previously synthesized nanoparticles, were produced by ionic crosslinking method based on modified ionic gelation of tripolyphosphate ( TPP) with GC-FUA. The effects of GC-FUA concerntration, TPP concerntration, pH value, etc. on the preparation of GC-FUA nanoparticles were evaluated. And results were when GC-FUA con-cerntration was 2. 0 mg/mL,TPP concerntration was 1. 0 mg/mL,pH value was 4. 5,GC-FUA/TPP mass ratio was 12∶1,opalescent solution of stable GC-FUA nanoparticles could be easily obtained. GC-FUA nanoparticles exhibited regularly spherical shapes, with a smooth surface and uniform size,and has a slow release properties which were confirmed by SEM and Nano ZS. Cytotoxicity study ( MTT) in HepG2 cell lines demonstrated that the resulting GC-FUA nanoparticles were more potent in killing cancer cells,compared to free 5-fluorouracil (5-Fu) and 5-Fu-loaded GC nanoparticles.