CAJ | 학술논문

目的：观察苓甘五味姜辛汤对寒饮伏肺型哮喘大鼠环腺苷酸(cAMP)、环腺苷酸依赖性蛋白激酶 A (PKA)及水通道蛋白5(AQP5)含量的影响,探讨其对寒饮伏肺型哮喘的作用机制。方法采用卵清蛋白腹腔注射和雾化激发＋寒冷刺激建立哮喘模型。将90只大鼠随机分为空白组、模型组、地塞米松组及苓甘五味姜辛汤低、中、高剂量组,各治疗组给予相应药物干预,ELISA检测大鼠cAMP、PKA、AQP5含量。结果与空白组比较,模型组大鼠cAMP、PKA、AQP5含量降低(P＜0.05)；与模型组比较,各治疗组大鼠cAMP、PKA、AQP5含量升高(P＜0.05),其中苓甘五味姜辛汤高剂量组作用显著(P＜0.05)。结论苓甘五味姜辛汤可调节寒饮伏肺型哮喘大鼠cAMP、PKA、AQP5正常分泌,增加气道液体分泌,降低黏蛋白浓度,从而起到治疗哮喘的作用。
목적：관찰령감오미강신탕대한음복폐형효천대서배선감산(cAMP)、배선감산의뢰성단백격매 A (PKA)급수통도단백5(AQP5)함량적영향,탐토기대한음복폐형효천적작용궤제。방법채용란청단백복강주사화무화격발＋한랭자격건립효천모형。장90지대서수궤분위공백조、모형조、지새미송조급령감오미강신탕저、중、고제량조,각치료조급여상응약물간예,ELISA검측대서cAMP、PKA、AQP5함량。결과여공백조비교,모형조대서cAMP、PKA、AQP5함량강저(P＜0.05)；여모형조비교,각치료조대서cAMP、PKA、AQP5함량승고(P＜0.05),기중령감오미강신탕고제량조작용현저(P＜0.05)。결론령감오미강신탕가조절한음복폐형효천대서cAMP、PKA、AQP5정상분비,증가기도액체분비,강저점단백농도,종이기도치료효천적작용。
Objective To observe the effects ofLinggan Wuwei Jiangxin Decoction on contents of cAMP, cAMP dependent PKA and AQP5 of model rats with clod phlegm-fluid lying latent in the lung asthma;To discuss its mechanism.Methods Ovalbumin intraperitoneal injection and atomization motivation + cold stimulation were used to establish models. Ninety rats were randomly divided into blank control group, model group, hexadecadrol group, andLinggan Wuwei Jiangxin Decoction low-, medium-, high-dose groups. All administration groups were treated with relevant medicine. The levels of cAMP, PKA, and AQP5 were detected by ELISA.Results Compared with the blank control group, the levels of cAMP, PKA, and AQP5 of rats in model group decreased obviously (P<0.05). Compared with the model group, the levels of cAMP, PKA, and AQP5 of rats in all administration groups increased (P<0.05), which increased dramatically inLinggan Wuwei Jiangxin Decoction high-dose group.ConclusionLinggan Wuwei Jiangxin Decoction can regulate the normal secretion of cAMP, PKA, and AQP5 in serum of rats with clod phlegm-fluid lying latent in the lung asthma, increase secretion of air passage liquid and decrease mucoprotein concentration, through which plays the role of treating asthma.