CAJ | 학술논문

目的：观察具有活血化瘀功效的溶栓胶囊对脑缺血缺氧耐受和脑储备能力影响。方法：采用左侧颈总动脉结扎并通入氧气和氮气（8?92）15 min的方法建立C57 BL/6小鼠急性脑缺血缺氧模型，造模后10 min十二指肠给药，给予不同剂量的溶栓胶囊（100、200、400 mg·kg-1）或生理盐水，评价局部脑血流量变化以及24 h后的神经行为学改变。SD大鼠给予不同剂量的溶栓胶囊（75、150、300 mg·kg-1）或生理盐水，观察溶栓胶囊对高分子右旋糖苷诱导的大鼠微循环障碍的改善作用。结果：与假手术组比较，模型组小鼠脑血流量明显下降，脑缺血范围增加，脑缺氧后行为学评分明显升高（P<0.05或P<0.01）；同时，模型组大鼠在注射高分子右旋糖苷后软脑膜血管出现微循环障碍，10、20、30 min 3个时间点大鼠软脑膜微循环血液流速明显减慢。与模型组比较，溶栓胶囊（400 mg·kg-1）可以明显增加小鼠局部脑血流量，改善行为学障碍，减小脑缺血范围（P<0.05或P<0.01）；溶栓胶囊还可改善高分子右旋糖苷诱导的大鼠软脑膜微血管不同时间点血液流速和流态（P<0.05或P<0.01）。结论：溶栓胶囊可通过增加脑缺血缺氧耐受和脑储备能力而达到保护缺血缺氧神经组织的作用，促进神经功能的恢复。
목적：관찰구유활혈화어공효적용전효낭대뇌결혈결양내수화뇌저비능력영향。방법：채용좌측경총동맥결찰병통입양기화담기（8?92）15 min적방법건립C57 BL/6소서급성뇌결혈결양모형，조모후10 min십이지장급약，급여불동제량적용전효낭（100、200、400 mg·kg-1）혹생리염수，평개국부뇌혈류량변화이급24 h후적신경행위학개변。SD대서급여불동제량적용전효낭（75、150、300 mg·kg-1）혹생리염수，관찰용전효낭대고분자우선당감유도적대서미순배장애적개선작용。결과：여가수술조비교，모형조소서뇌혈류량명현하강，뇌결혈범위증가，뇌결양후행위학평분명현승고（P<0.05혹P<0.01）；동시，모형조대서재주사고분자우선당감후연뇌막혈관출현미순배장애，10、20、30 min 3개시간점대서연뇌막미순배혈액류속명현감만。여모형조비교，용전효낭（400 mg·kg-1）가이명현증가소서국부뇌혈류량，개선행위학장애，감소뇌결혈범위（P<0.05혹P<0.01）；용전효낭환가개선고분자우선당감유도적대서연뇌막미혈관불동시간점혈액류속화류태（P<0.05혹P<0.01）。결론：용전효낭가통과증가뇌결혈결양내수화뇌저비능력이체도보호결혈결양신경조직적작용，촉진신경공능적회복。
This study was aimed to observe the effect ofRong-Shuan (RS) capsule on rodent tolerance against cerebral ischemia, hypoxia and cerebral reserve capacity, which was related to promote blood circulation and remove blood stasis. Acute cerebral ischemia and anoxia models were established by permanent left carotid artery ligation on C57 BL/6 mice and hypoxia inhalation (O2?N2 = 8?92) for 15 min. Duodenal administration of RS capsule at different doses (100, 200 or 400 mg·kg-1) or saline were given 10 min after ischemia onset. The local brain blood circulation changes and neurobehavioral function were evaluated 24 h after ischemia onset. SD rats were given RS capsule at different doses (75, 150, 300 mg·kg-1) or saline. The effect of RS capsule on improvement of microcirculation disturbance induced by high molecular dextran was observed. The results showed that compared with the sham operation group, the brain blood circulation in the model group was significantly decreased; the cerebral infarction area increased; and the behavioral score after cerebral hypoxia was significantly increased (P < 0.05, orP < 0.01). Meanwhile, after the injection of high molecular dextran among rats in the model group, the cerebral leptomeninx microcirculation was obviously slowed down at 3 timepoints, which were 10, 20 and 30 min. Compared with the model group, RS capsule (400 mg·kg-1) can significantly increase the local blood circulation in the brain of mice, improve behavioral disturbance, reduce cerebral ischemia area (P< 0.05, orP < 0.01). RS capsule can also improve blood flow velocity and flow pattern in cerebral leptomeninx microcirculation disturbance induced by high molecular dextran at different timepoints (P < 0.05, orP < 0.01). It was concluded that RS capsule can increase the tolerance against cerebral ischemia, hypoxia and cerebral reserve capacity in order to protect the neural tissues to promote neuronal recovery.