CAJ | 학술논문

目的：制备载脂联素缓释骨支架材料，并评价其体外生物学性能。方法：利用离子乳化交联法制备负载脂联素的壳聚糖微球，应用热致相分离法制备乳酸和乙醇酸共聚物／β-TCP 支架材料并在其中包覆载药微球。通过扫描电子显微镜、体外释放行为、检测材料浸提液对 MC3T3细胞凋亡和增殖作用等实验综合评价载药支架材料的性能及生物学活性。结果：微球直径均匀，载药支架孔径20～200μm 并相互穿通，载药率1．3％，包封率70．3％，在缓冲液中药物释放持续91 d。材料浸提液诱导细胞凋亡率与空白对照组相当，载药支架材料对成骨细胞的增殖有促进作用。结论：载脂联素缓释骨支架材料具有缓释效果并促进成骨细胞增殖。
목적：제비재지련소완석골지가재료，병평개기체외생물학성능。방법：이용리자유화교련법제비부재지련소적각취당미구，응용열치상분리법제비유산화을순산공취물／β-TCP 지가재료병재기중포복재약미구。통과소묘전자현미경、체외석방행위、검측재료침제액대 MC3T3세포조망화증식작용등실험종합평개재약지가재료적성능급생물학활성。결과：미구직경균균，재약지가공경20～200μm 병상호천통，재약솔1．3％，포봉솔70．3％，재완충액중약물석방지속91 d。재료침제액유도세포조망솔여공백대조조상당，재약지가재료대성골세포적증식유촉진작용。결론：재지련소완석골지가재료구유완석효과병촉진성골세포증식。
Objective:To prepare a bone substitute using microsphere scaffold containing adiponectin(APN)and to investigate the release behavior of the scaffold in vitro.Methods:Chitosan microsphere was developed by an emulsion-ionic cross-linking method. Poly (L-lactic-co-glycolic)acid (PLGA)and β-tricalcium phosphate (β-TCP)were used to prepare microsphere scaffold containing APN.The morphology,particle size,drug loading,incorporation efficiency and release behavior of the microsphere were examined. Results:The APN containing microsphere showed good spherical geometry,suitable size and microporosity under scanning electron microscope.The average diameter of the milipore was 20 -200 μm;the drug loading and incorporation efficiency were 1 .3% and 70.3% respectively.The controled-release process continued for 91 days.The extract solution from the APN microsphere-scaffold promoted MC3T3 cell proliferation without cytotoxicity.Conclusion:The APN microsphere-scaffold has sustained release function and may promote osteoblast proliferation.