CAJ | 학술논문

对四逆散的改善睡眠作用进行药理学评价，深入研究四逆散疏肝解郁功效的科学内涵。以大鼠、小鼠和果蝇为模式生物，利用行为药理学、皮层脑电描记与分析、生物化学及分子生物学等实验方法与技术，开展四逆散改善睡眠作用的药理评价。四逆散能延长戊巴比妥钠所致小鼠睡眠时间，其作用存在着量效与时效关系；能延长正常或睡眠剥夺大鼠的慢波睡眠时间；对正常或睡眠剥夺大鼠脑中的多种神经递质、睡眠相关因子等均有影响。四逆散具有改善睡眠的作用，该作用与其疏肝解郁功效密切相关，是通过多途径、多靶点来实现的。
대사역산적개선수면작용진행약이학평개，심입연구사역산소간해욱공효적과학내함。이대서、소서화과승위모식생물，이용행위약이학、피층뇌전묘기여분석、생물화학급분자생물학등실험방법여기술，개전사역산개선수면작용적약리평개。사역산능연장무파비타납소치소서수면시간，기작용존재착량효여시효관계；능연장정상혹수면박탈대서적만파수면시간；대정상혹수면박탈대서뇌중적다충신경체질、수면상관인자등균유영향。사역산구유개선수면적작용，해작용여기소간해욱공효밀절상관，시통과다도경、다파점래실현적。
Pharmacological evaluation on the improvement of sleeping effect usingSi-Ni-San(SNS) in order to conduct further study on scientific connotation of soothing liver-qistagnation. Rats, mice and fruit flies were used as animal model. Pharmacological assessment was conducted and done on the improvement of sleep effect by SNS with the cortical EEG scanning and analysis, biochemistry, molecular pharmacological methodology and technology. SNS can prolong the sleeping time of mice which were induced by pentobarbital sodium. Its effect showed the dose-dependent and time-dependent manner. It can prolong the slow-wave sleep time in normal and sleep deprivation rats. It influenced several neurotransmitters and endogenous sleep factors in brain of normal and sleep deprivation rats. SNS can improve sleeping. The effect was closely related the effect of soothing liver-qi stagnation. The function was realized through multiple pathways and targets.