世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
World Science and Technology-Modernization of Traditional Chinese Medicine
2015年
8期
1700-1706
,共7页
蓝婧%黄金珠%邓琳雯%王田平%王毅%曾倩
藍婧%黃金珠%鄧琳雯%王田平%王毅%曾倩
람청%황금주%산림문%왕전평%왕의%증천
补肾活血法外用%子宫内膜容受性%肾虚血瘀%子宫组织形态学
補腎活血法外用%子宮內膜容受性%腎虛血瘀%子宮組織形態學
보신활혈법외용%자궁내막용수성%신허혈어%자궁조직형태학
External method of tonifying kidney and promoting blood circulation%endometrial receptivity%kidney deficiency and blood stasis%histomorphology of uterus
目的：探讨补肾活血外治法对肾虚血瘀模型大鼠子宫内膜组织形态学的影响。方法：采用阴道涂片法选取处于动情前期普通级健康未交配雌性SD大鼠50只，随机分为空白组、模型组、补肾活血方高、低剂量组、五子组。除空白组外，其余各组采用羟基脲灌胃建造肾虚模型，与此同时，空白组、模型组给予蒸馏水灌肠，高、低剂量组分别给予高、低剂量补肾活血方药灌肠，五子组给予五子衍宗丸灌胃。妊娠第4天，各组大鼠均于末次给药后1 h，尾静脉快速推注10%的高分子右旋糖苷造成血瘀模型后处死，取子宫组织切片苏木精-伊红（HE）染色观察。结果：①模型组子宫内膜被覆上皮厚度低于空白组（P<0.05）；补肾活血方药高、低剂量组和五子组子宫内膜被覆上皮厚度与模型组相比有显著性差异（P<0.01）。②模型组腺体数、腺体面积总和及最大直径/最小直径明显低于空白组（P<0.05或P<0.01）；形状因子最接近“1”，与空白组相比有显著性差异（P<0.01）。补肾活血方高、低剂量组腺体数及腺腔面积总和较模型组有不同程度的增多（P<0.05或P<0.01）。五子组腺体数、腺腔面积总和、形状因子及最大直径/最小直径与模型组相比有显著性差异（P<0.05或P<0.01）。③模型组间质细胞核面积总和、间质细胞核积分光密度及间质血管数均低于空白组（P<0.01）；补肾活血方高、低剂量组间质细胞核面积总和、间质细胞核积分光密度和间质血管数较模型组均明显增高（P<0.05或P<0.01）；五子组间质细胞核面积总和及间质细胞核积分光密度明显高于模型组（P<0.01）。结论：外用补肾活血法能够有效改善肾虚血瘀模型大鼠子宫内膜组织形态，促使子宫内膜腺体与间质同步发育，从而发挥改善子宫内膜容受性的作用。
目的：探討補腎活血外治法對腎虛血瘀模型大鼠子宮內膜組織形態學的影響。方法：採用陰道塗片法選取處于動情前期普通級健康未交配雌性SD大鼠50隻，隨機分為空白組、模型組、補腎活血方高、低劑量組、五子組。除空白組外，其餘各組採用羥基脲灌胃建造腎虛模型，與此同時，空白組、模型組給予蒸餾水灌腸，高、低劑量組分彆給予高、低劑量補腎活血方藥灌腸，五子組給予五子衍宗汍灌胃。妊娠第4天，各組大鼠均于末次給藥後1 h，尾靜脈快速推註10%的高分子右鏇糖苷造成血瘀模型後處死，取子宮組織切片囌木精-伊紅（HE）染色觀察。結果：①模型組子宮內膜被覆上皮厚度低于空白組（P<0.05）；補腎活血方藥高、低劑量組和五子組子宮內膜被覆上皮厚度與模型組相比有顯著性差異（P<0.01）。②模型組腺體數、腺體麵積總和及最大直徑/最小直徑明顯低于空白組（P<0.05或P<0.01）；形狀因子最接近“1”，與空白組相比有顯著性差異（P<0.01）。補腎活血方高、低劑量組腺體數及腺腔麵積總和較模型組有不同程度的增多（P<0.05或P<0.01）。五子組腺體數、腺腔麵積總和、形狀因子及最大直徑/最小直徑與模型組相比有顯著性差異（P<0.05或P<0.01）。③模型組間質細胞覈麵積總和、間質細胞覈積分光密度及間質血管數均低于空白組（P<0.01）；補腎活血方高、低劑量組間質細胞覈麵積總和、間質細胞覈積分光密度和間質血管數較模型組均明顯增高（P<0.05或P<0.01）；五子組間質細胞覈麵積總和及間質細胞覈積分光密度明顯高于模型組（P<0.01）。結論：外用補腎活血法能夠有效改善腎虛血瘀模型大鼠子宮內膜組織形態，促使子宮內膜腺體與間質同步髮育，從而髮揮改善子宮內膜容受性的作用。
목적：탐토보신활혈외치법대신허혈어모형대서자궁내막조직형태학적영향。방법：채용음도도편법선취처우동정전기보통급건강미교배자성SD대서50지，수궤분위공백조、모형조、보신활혈방고、저제량조、오자조。제공백조외，기여각조채용간기뇨관위건조신허모형，여차동시，공백조、모형조급여증류수관장，고、저제량조분별급여고、저제량보신활혈방약관장，오자조급여오자연종환관위。임신제4천，각조대서균우말차급약후1 h，미정맥쾌속추주10%적고분자우선당감조성혈어모형후처사，취자궁조직절편소목정-이홍（HE）염색관찰。결과：①모형조자궁내막피복상피후도저우공백조（P<0.05）；보신활혈방약고、저제량조화오자조자궁내막피복상피후도여모형조상비유현저성차이（P<0.01）。②모형조선체수、선체면적총화급최대직경/최소직경명현저우공백조（P<0.05혹P<0.01）；형상인자최접근“1”，여공백조상비유현저성차이（P<0.01）。보신활혈방고、저제량조선체수급선강면적총화교모형조유불동정도적증다（P<0.05혹P<0.01）。오자조선체수、선강면적총화、형상인자급최대직경/최소직경여모형조상비유현저성차이（P<0.05혹P<0.01）。③모형조간질세포핵면적총화、간질세포핵적분광밀도급간질혈관수균저우공백조（P<0.01）；보신활혈방고、저제량조간질세포핵면적총화、간질세포핵적분광밀도화간질혈관수교모형조균명현증고（P<0.05혹P<0.01）；오자조간질세포핵면적총화급간질세포핵적분광밀도명현고우모형조（P<0.01）。결론：외용보신활혈법능구유효개선신허혈어모형대서자궁내막조직형태，촉사자궁내막선체여간질동보발육，종이발휘개선자궁내막용수성적작용。
This study aimed to analyze the effect of the external method of tonifying kidney and promoting blood circulation on endometrial morphology of rats with kidney deficiency and blood stasis model. A total of 50 normal healthy unmated female SD rats of proestrus were selected with vaginal smear. Rats were randomly divided into the blank group, model group, high dose group and low dose group with the prescription of tonifying kidney and promoting blood circulation and theWu-Zi (WZ) group. Except the blank group, intragastric administration of hydroxyurea was given to other group to establish the kidney deficiency model. Meanwhile, clyster of distilled water was given to the blank group and the model group. And clyster of high dose and low dose prescription of tonifying kidney and promoting blood circulation was given to the high dose and low dose group, respectively. The intragastric administrations ofWu-Zi Yan-Zong(WZYZ) pills were given to theWu-Zi (WZ) group. On the 4th day of pregnancy, 10% of the macromolecule dextran was quickly injected to the caudal vein to induce blood stasis model 1 hour after the last medication administration. The uterus tissue section was observed by HE dyeing. The results showed that the thickness of covering epithelium of endometrium in the model group was lower than the blank group (P < 0.05). There were significant differences on the thickness of covering epithelium of endometrium in the high dose group and low dose group with the prescription of tonifying kidney and promoting blood circulation, the WZ group and the model group (P < 0.01). The sum of gland number and gland area as well as the maximum diameter / minimum diameter of the model group was significantly lower than that of the blank group (P< 0.05, orP < 0.01). The shape factor was the closest to “1”, which had significant difference compared with the blank group (P < 0.01). Compared with the model group, the sum of gland number and glandular lumen of the high dose and low dose group were increased at different levels (P < 0.05, orP < 0.01). The sum of gland number, glandular lumen area, shape factor, and maximum diameter / minimum diameter of WZ group had significant difference compared with the model group (P < 0.05, orP < 0.01). The sum of interstitial cells’ nucleus area, integral optical density of interstitial cells’ nucleus and number of interstitial blood vessels in the high dose and low dose group were significantly higher compared to the model group (P < 0.05, orP < 0.01). The sum of interstitial cells’ nucleus area and the integral optical density of interstitial cells’ nucleus in the WZ group were obviously higher than the model group (P < 0.01). It was concluded that the external method of tonifying kidney and promoting blood circulation can effectively improve the endometrial morphology of kidney-deficiency and blood-stasis rat model, promote the synchronous development of endometrial gland and stroma, in order to play a role to improve the endometrial receptivity.
