CAJ | 학술논문

目的：探讨半乳凝素-7(Galectin-7)在维吾尔族与汉族妇女子宫颈鳞状细胞癌和子宫颈炎中的表达,并分析其与HPV 16感染、子宫颈癌分化程度、患病年龄的相关性。方法采用免疫组化SP法分别检测Galectin-7在维吾尔族21例子宫颈炎和49例子宫颈鳞状细胞癌、汉族25例子宫颈炎和29例子宫颈鳞状细胞癌中的表达；采用PCR技术检测HPV 16感染情况。利用图像分析软件Image-Pro Plus(IPP)6．0半定量检测子宫颈病变中Galectin-7的累计光密度(integrated optical density, IOD)值。结果 PCR检测显示维吾尔族子宫颈鳞状细胞癌中HPV 16阳性者20例,阴性者29例；子宫颈炎中HPV 16阳性者5例,阴性者16例。汉族子宫颈癌中HPV 16阳性者2例,阴性者27例；子宫颈炎中HPV 16均阴性。 Galectin-7在维吾尔族子宫颈鳞状细胞癌中的表达低于子宫颈炎组织(P<0.05),其在维吾尔族HPV 16阳性子宫颈鳞状细胞癌组织中的表达低于HPV 16阴性组织(P<0．05)。 Galectin-7表达与子宫颈鳞状细胞癌分化程度、患病年龄无相关性(P>0．05)。结论维吾尔族子宫颈鳞状细胞癌与子宫颈炎组织相比,Galectin-7表达量下降；另外其在维吾尔族HPV 16阳性子宫颈鳞状细胞癌中的表达低于HPV 16阴性者,这可能与子宫颈鳞状细胞癌的发生、发展、浸润、转移密切相关,检测其表达对维吾尔族妇女子宫颈癌诊断有一定的参考价值。
목적：탐토반유응소-7(Galectin-7)재유오이족여한족부녀자궁경린상세포암화자궁경염중적표체,병분석기여HPV 16감염、자궁경암분화정도、환병년령적상관성。방법채용면역조화SP법분별검측Galectin-7재유오이족21례자궁경염화49례자궁경린상세포암、한족25례자궁경염화29례자궁경린상세포암중적표체；채용PCR기술검측HPV 16감염정황。이용도상분석연건Image-Pro Plus(IPP)6．0반정량검측자궁경병변중Galectin-7적루계광밀도(integrated optical density, IOD)치。결과 PCR검측현시유오이족자궁경린상세포암중HPV 16양성자20례,음성자29례；자궁경염중HPV 16양성자5례,음성자16례。한족자궁경암중HPV 16양성자2례,음성자27례；자궁경염중HPV 16균음성。 Galectin-7재유오이족자궁경린상세포암중적표체저우자궁경염조직(P<0.05),기재유오이족HPV 16양성자궁경린상세포암조직중적표체저우HPV 16음성조직(P<0．05)。 Galectin-7표체여자궁경린상세포암분화정도、환병년령무상관성(P>0．05)。결론유오이족자궁경린상세포암여자궁경염조직상비,Galectin-7표체량하강；령외기재유오이족HPV 16양성자궁경린상세포암중적표체저우HPV 16음성자,저가능여자궁경린상세포암적발생、발전、침윤、전이밀절상관,검측기표체대유오이족부녀자궁경암진단유일정적삼고개치。
Purpose To study the expression of Galectin-7 in squamous carcinoma of the cervix and its correlation with HPV 16 infec-tion in Uyghur and Han women. Methods The expression of Galectin-7 in paraffin-embedded cervical tissues collected from Uyghur women including 21 patients with cervicitis and 49 patients with squamous carcinoma of the cervix and Han women including 25 patients with cervicitis and 29 patients with squamous cervical cancer was evaluated by immunohistochemical SP method. The infection of HPV 16 was evaluated by PCR amplification of HPV DNA from the corresponding cervical tissue samples. The expression of levels of Galec-tin-7 in cervical tissues was analyzed according to the corresponding integrated optical density ( IOD) value of Galectin-7 in cervical le-sions with professional image analysis software Image-Pro Plus (IPP) 6. 0. Results PCR detection of HPV 16 in Uyghur patients from Kashghar showed that 20 patients were positive and 29 cases were negative for HPV 16 in squamous cervical cancer patients whereas in patients with cervicitis, only 5 cases were positive, 16 cases were negative for HPV 16. Furthermore the detection of HPV 16 in Han patients showed that 2 cases were positive and 27 cases was negative in patients with squamous cervical cancer patients, and all the ca-ses were negative for HPV 16 in patients with cervicitis. The expression of Galectin-7 in squamous cervical cancer tissues was signifi-cantly lower than that in the cervicitis patients (P<0. 05). The expression of Galectin-7 in HPV 16 positive squamous cervical cancer patients was significantly lower than that in HPV 16 negative patients ( P<0. 05 ) . There was no obvious correlation between the ex-pression levels of Galectin-7 in squamous cervical cancer patients with the differentiation degree and disease oneset age both in Uyghur and Han patients. Conclusion Compared with cervicitis tissues, Galectin-7 expression in squamous cervical cancer is down-regulated in Uyghur women from Kashghar region implying that the expression of Galectin-7 may play a certain role in the occurrence and develop-ment of squamous cervical cancer, and its detection has a certain reference value for the diagnosis of cervical cancer for Uyghur women.