临床与实验病理学杂志
臨床與實驗病理學雜誌
림상여실험병이학잡지
Chinese Journal of Clinical and Experimental Pathology
2015年
9期
1009-1012
,共4页
姜维娜%黄维清%刘鑫%刘红云%孔庆暖%韩增磊%陈桦
薑維娜%黃維清%劉鑫%劉紅雲%孔慶暖%韓增磊%陳樺
강유나%황유청%류흠%류홍운%공경난%한증뢰%진화
结肠肿瘤%OTUB1%实时定量PCR%免疫组织化学
結腸腫瘤%OTUB1%實時定量PCR%免疫組織化學
결장종류%OTUB1%실시정량PCR%면역조직화학
colon neoplasm%OTUB1%quantitative real-time PCR%immunohistochemistry
目的：探讨OTUB1在结肠癌组织中的表达及其临床意义。方法采用实时定量PCR和免疫组化SP法检测OTUB1在结肠癌组织和癌旁组织中的表达。结果 OTUB1 mRNA在结肠癌组织中的表达较癌旁组织高3．5倍，癌组织中OTUB1的蛋白表达水平也明显高于癌旁组织(P<0．05)，且呈现癌旁组织、腺瘤、结肠癌逐渐递增的趋势(P<0．05)。 OTUB1高表达与肿瘤大小、分化程度和淋巴结转移密切相关。结论 OTUB1在结肠癌的发生、发展过程中发挥重要作用。
目的：探討OTUB1在結腸癌組織中的錶達及其臨床意義。方法採用實時定量PCR和免疫組化SP法檢測OTUB1在結腸癌組織和癌徬組織中的錶達。結果 OTUB1 mRNA在結腸癌組織中的錶達較癌徬組織高3．5倍，癌組織中OTUB1的蛋白錶達水平也明顯高于癌徬組織(P<0．05)，且呈現癌徬組織、腺瘤、結腸癌逐漸遞增的趨勢(P<0．05)。 OTUB1高錶達與腫瘤大小、分化程度和淋巴結轉移密切相關。結論 OTUB1在結腸癌的髮生、髮展過程中髮揮重要作用。
목적：탐토OTUB1재결장암조직중적표체급기림상의의。방법채용실시정량PCR화면역조화SP법검측OTUB1재결장암조직화암방조직중적표체。결과 OTUB1 mRNA재결장암조직중적표체교암방조직고3．5배，암조직중OTUB1적단백표체수평야명현고우암방조직(P<0．05)，차정현암방조직、선류、결장암축점체증적추세(P<0．05)。 OTUB1고표체여종류대소、분화정도화림파결전이밀절상관。결론 OTUB1재결장암적발생、발전과정중발휘중요작용。
Purpose To investigate the expression of OTUB1 in colon cancer and the relationship between its expression and some clinicopathologic parameters. Methods Quantitative real-time PCR and immunohistochemical SP method were carried out in selected colon cancer and normal mucosa tissues. Results OTUB1 mRNA in colon cancer was 3. 5-fold higher than the normal mucosa. The expression of OTUB1 protein in the colon cancer was significantly higher than normal mucosa (P<0. 05). Moreover, its expression in normal tissues, adenoma and colon cancer showed a gradually increasing trend (P<0. 05). The higher expression of OTUB1 in colon cancer was related with tumor size, differentiation and lymph node metastasis. Conclusions OTUB1 may play an important role in co-lon cancer development.
