CAJ | 학술논문

目的：探讨PTEN、p53和上皮生长因子受体( epidermal growth factor receptor, EGFR)在乳腺癌中表达的临床病理意义及其相关性。方法采用免疫组化MaxVision两步法检测209例乳腺浸润性导管癌和40例良性乳腺病变中PTEN、p53、EGFR蛋白的表达。结果 PTEN、p53和EGFR在乳腺癌中的过表达率为57．9%、55．0%和38．3%,与良性乳腺病变间差异有显著性(P均<0．05)。 PTEN、p53及EGFR蛋白表达均与肿瘤大小、组织学分级、淋巴结转移、ER和PR状态、分子分型有显著相关性(P均<0．05)；PTEN、p53表达与TNM分期有显著相关性；PTEN、EGFR表达与HER-2状态有显著相关性。 EGFR、p53表达与PTEN呈显著负相关(P均<0．01),p53表达与EGFR呈显著正相关(P=0．000)。 PTEN、EGFR表达与乳腺癌预后相关,但不是独立的预后因子。具有PTEN-/p53+/EGFR+组合表达的乳腺癌生存率低于其他组合(P=0．008)。结论 PTEN低或失表达、p53突变和EGFR过表达可能协同参与乳腺癌的发生、发展,三者联合检测对乳腺癌的治疗和预后可能具有指导作用。
목적：탐토PTEN、p53화상피생장인자수체( epidermal growth factor receptor, EGFR)재유선암중표체적림상병리의의급기상관성。방법채용면역조화MaxVision량보법검측209례유선침윤성도관암화40례량성유선병변중PTEN、p53、EGFR단백적표체。결과 PTEN、p53화EGFR재유선암중적과표체솔위57．9%、55．0%화38．3%,여량성유선병변간차이유현저성(P균<0．05)。 PTEN、p53급EGFR단백표체균여종류대소、조직학분급、림파결전이、ER화PR상태、분자분형유현저상관성(P균<0．05)；PTEN、p53표체여TNM분기유현저상관성；PTEN、EGFR표체여HER-2상태유현저상관성。 EGFR、p53표체여PTEN정현저부상관(P균<0．01),p53표체여EGFR정현저정상관(P=0．000)。 PTEN、EGFR표체여유선암예후상관,단불시독립적예후인자。구유PTEN-/p53+/EGFR+조합표체적유선암생존솔저우기타조합(P=0．008)。결론 PTEN저혹실표체、p53돌변화EGFR과표체가능협동삼여유선암적발생、발전,삼자연합검측대유선암적치료화예후가능구유지도작용。
Purpose To study clinical pathological significance of the expression of PTEN, p53 and epidermal growth factor receptor ( EGFR) in breast carcinoma and their correlation. Methods Immunohistochemical MaxVision method was used to detect the expres-sion of PTEN, p53, EGFR in 209 cases of infiltrating ductal carcinoma of breast and 40 cases of benign breast diseases. Results The over-expression rate of PTEN, p53 and EGFR protein in breast carcinoma was 57. 9%, 55. 0% and 38. 3% respectively, which was significantly different from those in benign breast diseases (P<0. 05). The expression of PTEN, p53 and EGFR in breast cancer was correlated with tumor size, histological grade, lymph node metastasis, ER status, PR status and molecular subtype (P<0. 05). There was an association between PTEN or p53 and TNM stage, PTEN or EGFR and HER-2 status. There was a negative correlation in the protein expression of PTEN vs EGFR or PTEN vs p53 (P<0. 01). There was a positive correlation between EGFR and p53 protein ex-pression (P=0. 000). The expression of PTEN or EGFR was correlated with the prognosis of breast cancer, but not independent prog-nostic factors. The survival rate of patients with PTEN- /p53 + /EGFR+ was lower than those with other combined expression of PTEN/p53/EGFR (P=0. 008). Conclusions Low or loss expression of PTEN, p53 mutation and EGFR over-expression may be coordinate-ly involved in the pathogenesis and progression of breast cancer. The combined detection of these markers may play an important role in making treatment and indicating prognosis for breast cancer patients.