CAJ | 학술논문

目的：探讨黄芩提取物对肝癌H22模型小鼠的抑瘤作用及其可能的作用机制。方法将H22瘤株接种于小鼠，建立移植瘤模型。造模成功后随机分为模型组、5-Fu组及黄芩提取物低、中、高剂量组，另取12只正常小鼠作为对照组。各给药组给予相应药物灌胃，观察给药后小鼠体质量，计算肿瘤抑制率、胸腺及脾脏指数，分光光度法检测血清总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量，单细胞凝胶电泳检测外周血淋巴细胞DNA损伤。结果黄芩提取物中、高剂量对肿瘤生长有显著抑制作用。与5-Fu组比较，黄芩提取物中、高剂量组能显著提高小鼠体质量、胸腺和脾脏指数，提高血清T-AOC、SOD活性，降低MDA含量，减轻外周血淋巴细胞DNA损伤。结论黄芩提取物具有明显的抗肿瘤作用，其作用机制可能与减轻肿瘤对机体正常组织细胞的伤害、修复或增强小鼠的免疫功能，以及提高机体抗氧化损伤能力有关。
목적：탐토황금제취물대간암H22모형소서적억류작용급기가능적작용궤제。방법장H22류주접충우소서，건립이식류모형。조모성공후수궤분위모형조、5-Fu조급황금제취물저、중、고제량조，령취12지정상소서작위대조조。각급약조급여상응약물관위，관찰급약후소서체질량，계산종류억제솔、흉선급비장지수，분광광도법검측혈청총항양화능력(T-AOC)、초양화물기화매(SOD)활성급병이철(MDA)함량，단세포응효전영검측외주혈림파세포DNA손상。결과황금제취물중、고제량대종류생장유현저억제작용。여5-Fu조비교，황금제취물중、고제량조능현저제고소서체질량、흉선화비장지수，제고혈청T-AOC、SOD활성，강저MDA함량，감경외주혈림파세포DNA손상。결론황금제취물구유명현적항종류작용，기작용궤제가능여감경종류대궤체정상조직세포적상해、수복혹증강소서적면역공능，이급제고궤체항양화손상능력유관。
Objective To study the antitumor effects of Scutellaria Radix extracts on H22 transplanted tumor in mice and its possible mechanism.Methods The H22 transplanted mouse models were established by inoculating H22 to mice. 60 mice were randomly divided into model group, 5-Fu group, low-, medium- and high-dose Scutellaria Radix extracts groups, with 12 normal mice as control group. All administration groups received gavage with relevant medicine. And then the body weight change, tumor growth inhibitory rate, and spleen and thymus indexes were calculated;the T-AOC and activities of SOD, and MDA content in serum were detected by spectrophotometer;DNA damage of peripheral blood lymphocytes was observed by single cell gel electrophoresis.Results Medium- and high-dose Scutellaria Radix extracts can significantly inhibit the growth of transplanted tumor. Compared with 5-Fu group, medium- and high-dose Scutellaria Radix extracts groups notably increased the body weight, spleen and thymus indexes of mice, promoted T-AOC and activities of SOD, and decreased MDA content in serum, as well as notably reduced DNA damage of peripheral blood lymphocytes.Conclusion Scutellaria Radix extracts have obvious antitumor effects on H22 transplanted tumor in mice and the possible mechanisms may be due to lightening the damage of normal tissue and cell from tumor, enhancing immunologic function and improving antioxidant capability of H22 bearing mice.