临床与实验病理学杂志
臨床與實驗病理學雜誌
림상여실험병이학잡지
Chinese Journal of Clinical and Experimental Pathology
2015年
9期
991-995
,共5页
周炳娟%孙吉瑞%赵文明%陈雪%乔海芝%张丙信%李金梅%张金库
週炳娟%孫吉瑞%趙文明%陳雪%喬海芝%張丙信%李金梅%張金庫
주병연%손길서%조문명%진설%교해지%장병신%리금매%장금고
乳腺肿瘤%分子亚型%HIF-1α%Glut-1
乳腺腫瘤%分子亞型%HIF-1α%Glut-1
유선종류%분자아형%HIF-1α%Glut-1
breast neoplasm%molecular subtypes%HIF-1α%Glut-1
目的:探讨乳腺癌各分子亚型中HIF-1α和Glut-1的表达及其与基底细胞样型乳腺癌的关系。方法回顾性分析803例浸润性导管癌的临床病理资料及 ER、PR、HER-2的表达,采用免疫组化 MaxVision法检测 CK5/6和 EGFR的表达,按照Nielsen标准将乳腺癌分成Luminal A型、Luminal B型、HER-2过表达型、基底细胞样型、普通乳腺样型;同时检测HIF-1α和Glut-1在各型乳腺癌中的表达。结果在乳腺癌基底细胞样型、HER-2过表达型、普通乳腺样型、Luminal A型、Luminal B型中,HIF-1α的阳性率分别为77.89%(74/95)、56.06%(37/66)、55.76%(92/165)、31.97%(141/441)、25.00%(9/36);Glut-1的阳性率分别为80.00%(76/95)、57.58%(38/66)、58.18%(96/165)、34.01%(150/441)、25.00%(9/36)。 HIF-1α及Glut-1在基底细胞样型、HER-2过表达型和普通乳腺样型中的表达显著高于Luminal A型和Luminal B型( P<0.0045),且与ER表达呈显著负相关(P<0.01);ER阴性乳腺癌中,HIF-1α及Glut-1在基底细胞样型中的阳性率显著高于其它型(P<0.0045),且二者的表达呈显著正相关(P<0.01)。结论 HIF-1α和Glut-1高表达与ER阴性乳腺癌关系密切,且可能在基底细胞样型乳腺癌的发生、发展中起重要作用。
目的:探討乳腺癌各分子亞型中HIF-1α和Glut-1的錶達及其與基底細胞樣型乳腺癌的關繫。方法迴顧性分析803例浸潤性導管癌的臨床病理資料及 ER、PR、HER-2的錶達,採用免疫組化 MaxVision法檢測 CK5/6和 EGFR的錶達,按照Nielsen標準將乳腺癌分成Luminal A型、Luminal B型、HER-2過錶達型、基底細胞樣型、普通乳腺樣型;同時檢測HIF-1α和Glut-1在各型乳腺癌中的錶達。結果在乳腺癌基底細胞樣型、HER-2過錶達型、普通乳腺樣型、Luminal A型、Luminal B型中,HIF-1α的暘性率分彆為77.89%(74/95)、56.06%(37/66)、55.76%(92/165)、31.97%(141/441)、25.00%(9/36);Glut-1的暘性率分彆為80.00%(76/95)、57.58%(38/66)、58.18%(96/165)、34.01%(150/441)、25.00%(9/36)。 HIF-1α及Glut-1在基底細胞樣型、HER-2過錶達型和普通乳腺樣型中的錶達顯著高于Luminal A型和Luminal B型( P<0.0045),且與ER錶達呈顯著負相關(P<0.01);ER陰性乳腺癌中,HIF-1α及Glut-1在基底細胞樣型中的暘性率顯著高于其它型(P<0.0045),且二者的錶達呈顯著正相關(P<0.01)。結論 HIF-1α和Glut-1高錶達與ER陰性乳腺癌關繫密切,且可能在基底細胞樣型乳腺癌的髮生、髮展中起重要作用。
목적:탐토유선암각분자아형중HIF-1α화Glut-1적표체급기여기저세포양형유선암적관계。방법회고성분석803례침윤성도관암적림상병리자료급 ER、PR、HER-2적표체,채용면역조화 MaxVision법검측 CK5/6화 EGFR적표체,안조Nielsen표준장유선암분성Luminal A형、Luminal B형、HER-2과표체형、기저세포양형、보통유선양형;동시검측HIF-1α화Glut-1재각형유선암중적표체。결과재유선암기저세포양형、HER-2과표체형、보통유선양형、Luminal A형、Luminal B형중,HIF-1α적양성솔분별위77.89%(74/95)、56.06%(37/66)、55.76%(92/165)、31.97%(141/441)、25.00%(9/36);Glut-1적양성솔분별위80.00%(76/95)、57.58%(38/66)、58.18%(96/165)、34.01%(150/441)、25.00%(9/36)。 HIF-1α급Glut-1재기저세포양형、HER-2과표체형화보통유선양형중적표체현저고우Luminal A형화Luminal B형( P<0.0045),차여ER표체정현저부상관(P<0.01);ER음성유선암중,HIF-1α급Glut-1재기저세포양형중적양성솔현저고우기타형(P<0.0045),차이자적표체정현저정상관(P<0.01)。결론 HIF-1α화Glut-1고표체여ER음성유선암관계밀절,차가능재기저세포양형유선암적발생、발전중기중요작용。
Purpose To study the expression of HIF-1αand Glut-1 in the molecular subtypes of breast carcinoma and their correlation with basal-like breast carcinoma. Methods 803 cases of invasive breast carcinoma from our database were identified. The clinicopath-ologic findings and the biologic markers including estrogen receptor ( ER) , progesterone receptor ( PR) , and human epidermal growth factor receptor-2 (HER-2) status were reviewed. Immunohistochemical MaxVision stains for cytokeratin 5/6 (CK5/6) and epidermal growth factor receptor ( EGFR) were performed. All breast carcinomas were subclassified into Luminal A, Lumincal B, HER-2 over-expression, normal-like, and basal-like subtypes according to Nielsen criteria. Immunohistochemical stain was also used to detect the expression of HIF-1αand Glut-1. Results Positive expression rates of HIF-1αprotein in basal-like, HER-2 over-expression, normal-like, Luminal A and Luminal B substypes were 77. 89% (74/95), 56. 06% (37/66), 55. 76% (92/165), 31. 97% (141/441), 25. 00% (9/36), respectively. The positive expression rates of Glut-1 protein were 80. 00% (76/95), 57. 58% (38/66), 58. 18%(96/165), 34. 01% (150/441), 25. 00% (9/36), respectively. The positive expression rates of HIF-1α and Glut-1 in the basal-like, HER-2 over-expressing and normal-like subtypes were remarkably higher than that in Luminal A and Luminal B subtypes ( P<0. 004 5) and the expression of HIF-1a and Glut-1 was negatively correlated with the expression of ER (P<0. 01). In the ER-negative breast cancers, the positive expression rates of HIF-1a and Glut-1 in basal-like substype were much higher than that in the other sub-types (P<0. 004 5), and the expression of HIF-1α was positively correlated with expression of Glut-1 in basal-like breast carcinoma (P<0. 01). Conclusion The overexpression of HIF-1αand Glut-1 may be closely related to the ER-negative breast cancer and HIF-1α and Glut-1 might play an important role in the development of basal-like breast carcinoma.