CAJ | 학술논문

目的：了解郑州大学附属肿瘤医院临床和环境中产超广谱β-内酰胺酶（ ESBLs）大肠埃希菌多位点序列分型（ MLST）及其遗传进化关系，为监测产ESBLs菌株的流行和医院感染控制提供依据。方法采用基因扩增和测序的方法对26株产ESBLs大肠埃希菌7个管家基因进行序列分析，并与MLST数据库比对，得到每个菌株相应的等位基因号。结果26株产ESBLs大肠埃希菌获得16种序列分型（ ST），其中3株为ST69型、3株为ST131型。结论产ESBLs大肠埃希菌遗传背景多样化，ST131型和ST69型为本研究主要型别，其它型别散在分布。
목적：료해정주대학부속종류의원림상화배경중산초엄보β-내선알매（ ESBLs）대장애희균다위점서렬분형（ MLST）급기유전진화관계，위감측산ESBLs균주적류행화의원감염공제제공의거。방법채용기인확증화측서적방법대26주산ESBLs대장애희균7개관가기인진행서렬분석，병여MLST수거고비대，득도매개균주상응적등위기인호。결과26주산ESBLs대장애희균획득16충서렬분형（ ST），기중3주위ST69형、3주위ST131형。결론산ESBLs대장애희균유전배경다양화，ST131형화ST69형위본연구주요형별，기타형별산재분포。
Objective To investigate the multilocus sequence typing ( MLST) and genetic evolution of extended-spectrum beta-lactamase ( ESBLs)-producing Escherichia coli in the Affiliated Cancer Hospital of Zhengzhou University, and to provide the reference for the monitoring of ESBLs-producing isolates′epidemic and hospital infection control. Methods Gene amplification and sequence analysis were performed for 7 housekeeping genes of 26 isolates of ESBLs-producing Escherichia coli, and alleles were compared with those assigned at MLST databases.Results A total of 16 sequence types ( ST) were obtained in the 26 isolates of ESBLs-producing Escherichia coli, in which there were 3 ST69 and 3 ST131.Conclusions ESBLs-producing Escherichia coli has diversified genetic backgrounds, and ST131 and ST69 are the most prevalent types, while other types were scattered.