CAJ | 학술논문

目的：检测葫芦素E诱导刚地弓形虫从感染的宿主细胞逸出的效力。方法用不同浓度（20、50和100μmol／L）的葫芦素E孵育刚地弓形虫感染的HFF细胞1 h，统计纳虫空泡破损比例；用细胞松弛素D、1，2-二（2-氨基苯氧基）乙烷-N，N，N＇，N＇-四乙酸四乙酰氧甲基酯（ BAPTA-AM）预处理感染刚地弓形虫的HFF细胞，用50μmol／L葫芦素E孵育1 h，统计纳虫空泡破损比例；将逸出虫体重新感染细胞或腹腔注射昆明小鼠，检测虫体毒力变化。结果葫芦素E可以诱导刚地弓形虫从HFF细胞释放逸出，葫芦素E浓度越高，逸出率越高。葫芦素E诱导的虫体逸出与虫体的运动能力和虫体内钙离子有关，逸出虫体的毒力没有发生明显变化。结论葫芦素E可以诱导刚地弓形虫的逸出，为探索弓形虫和宿主细胞相互作用提供了新的研究方向。
목적：검측호호소E유도강지궁형충종감염적숙주세포일출적효력。방법용불동농도（20、50화100μmol／L）적호호소E부육강지궁형충감염적HFF세포1 h，통계납충공포파손비례；용세포송이소D、1，2-이（2-안기분양기）을완-N，N，N＇，N＇-사을산사을선양갑기지（ BAPTA-AM）예처리감염강지궁형충적HFF세포，용50μmol／L호호소E부육1 h，통계납충공포파손비례；장일출충체중신감염세포혹복강주사곤명소서，검측충체독력변화。결과호호소E가이유도강지궁형충종HFF세포석방일출，호호소E농도월고，일출솔월고。호호소E유도적충체일출여충체적운동능력화충체내개리자유관，일출충체적독력몰유발생명현변화。결론호호소E가이유도강지궁형충적일출，위탐색궁형충화숙주세포상호작용제공료신적연구방향。
Objective To investigate the effect of cucurbitacin E on the egress of Toxoplasma gondii from host cells.Methods Toxoplasma gondii infected HFF cells were treated with cucurbitacin E (20,50 and 100 μmol/L) for 1 h, and the egress rate was evaluated statistically.Toxoplasma gondii infected HFF cells were pretreated with Cyto-D and 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis acetoxymethyl ester(BAPTA-AM), followed by 50 μmol/L cucurbitacin E treatment for 1 h and egress determination.The virulence change of egressing Toxoplasma gondii, such parasites were collected and used to re-infect HFF or infect Kunming mice, was analyzed.Results Cucurbitacin E could induce the egress of Toxoplasma gondii from host cells, and the egress rate increased with the concentration of cucurbitaci E.Cucurbitacin E-induced ergess depended on the gliding ability of Toxoplasma gondii and intra-parasite calcium. There was no difference between cucurbitacin E-induced egressing parasite and naturally egressing parasite in virulence.Conclusions Cucurbitacin E could induce the egress of Toxoplasma gondii from host cells, which provides new direction to study the interactions between Toxoplasma gondii and its host cells.