CAJ | 학술논문

目的：以同时测定防风中的两种色原酮物质升麻素苷和5-O-甲基维斯阿米醇苷为目标，建立了上述两种物质的的含量测定方法。方法：色谱柱采用 Agilent TC-C18（250 mm ×4．6 mm，5μm）；梯度洗脱的流动相为水、甲醇，检测波长254 nm，流速1 mL／min，柱温35℃，进样量10μL。结果：不同生长方式的防风样品的5-O-甲基维斯阿米醇苷含量无统计学意义，野生和栽培防风的升麻素苷含量有差异，不同生长方式对防风样品的色原酮含量有差异。结论：野生防风质量较好，同时测定两种色原酮方法简便，结果准确，精密度、重现性、稳定性较好，可以此作为防风中升麻素苷和5-O-甲基维斯阿米醇苷的测定方法。
목적：이동시측정방풍중적량충색원동물질승마소감화5-O-갑기유사아미순감위목표，건립료상술량충물질적적함량측정방법。방법：색보주채용 Agilent TC-C18（250 mm ×4．6 mm，5μm）；제도세탈적류동상위수、갑순，검측파장254 nm，류속1 mL／min，주온35℃，진양량10μL。결과：불동생장방식적방풍양품적5-O-갑기유사아미순감함량무통계학의의，야생화재배방풍적승마소감함량유차이，불동생장방식대방풍양품적색원동함량유차이。결론：야생방풍질량교호，동시측정량충색원동방법간편，결과준학，정밀도、중현성、은정성교호，가이차작위방풍중승마소감화5-O-갑기유사아미순감적측정방법。
Objective:To establish a content determination method to simultaneously determine two kinds of chromoneS including Prim-O-glucosylcimifugin and 4’-O-beta-Glucopyranosyl-5-O-Methylvisamminol in Fangfeng.Methods:Agilent TC-C18 (250 mm ×4.6 mm,5 μm)was used as chromatographic column;water and methanol were used as gradient elution of the mobile phase, wave length of 254nm was detected,with the flow rate of 1 mL /min,column temperature of 35 ℃,the injection volume 10μL. Results:4’-O-beta-Glucopyranosyl-5-O-Methylvisamminol content of Fangfeng with different growth patterns had no significant difference;there was difference in the content of Prim-O-glucosylcimifugin between wild and cultivated Fangfeng;there were differ-ences in Chromones contents in Fangfeng with different growth patterns.Conclusion:Wild Fangfeng has better quality,and the simultaneous determination of two Chromones method is simple,accurate,with good precision,reproducibility and stability,and this can be used as determination method of Prim-O-glucosylcimifugin and 4 ’-O-beta-Glucopyranosyl-5-O-Methylvisamminol in Fangfeng.