世界中医药
世界中醫藥
세계중의약
World Chinese Medicine
2015年
9期
1387-1390
,共4页
陈红英%李贤玉%王文杰%彭吉霞
陳紅英%李賢玉%王文傑%彭吉霞
진홍영%리현옥%왕문걸%팽길하
大鼠急性心肌缺血%参麦%血管内皮生长因子%缺氧诱导因子-1%受体胎肝激酶-1
大鼠急性心肌缺血%參麥%血管內皮生長因子%缺氧誘導因子-1%受體胎肝激酶-1
대서급성심기결혈%삼맥%혈관내피생장인자%결양유도인자-1%수체태간격매-1
Acute myocardial ischemia of rats%Shenmai injection%Vascular endothelial growth factor%Hypoxia inducible factor-1%receptor in fetal liver kinase-1
目的：观察参麦对大鼠急性心肌缺血血管内皮生长因子表达的影响，探讨其作用机制。方法：大鼠40只，采用结扎左侧冠状动脉前降支30 min 后松解扎扣再灌注60 min，反复3次以造成心肌缺血再灌注损伤模型，并随机分为对照组、模型组、美托洛尔组及参麦小大剂量组。五组大鼠均经腹腔注射给药，于治疗前及30 d 后分别采用反转录-聚合酶链反应（RT-PCR）和免疫组织化学检测心肌 VEGF 和缺氧诱导因子-1a（HIF-1a）HIF-la 表达情况、受体胎肝激酶1（FLK-1），籍此考察参麦对大鼠急性心肌缺血血管内皮生长因子表达的影响。结果：参麦明显增高 VEGF 蛋白质及 mRNA、HIF-1a、FLK-1的表达，缩小心肌缺血面积，与模型组比较（P ＜0．05），差异均有统计学意义，参麦小、大剂量组间差异无统计学意义。结论：参麦可减轻大鼠急性心肌缺血缺氧性损伤和血管内皮炎症反应程度，其作用机制可能是其促进大鼠缺血心肌血管内皮再生有关，对改善再灌注损伤血管内皮功能紊乱有一定的防治作用。
目的：觀察參麥對大鼠急性心肌缺血血管內皮生長因子錶達的影響，探討其作用機製。方法：大鼠40隻，採用結扎左側冠狀動脈前降支30 min 後鬆解扎釦再灌註60 min，反複3次以造成心肌缺血再灌註損傷模型，併隨機分為對照組、模型組、美託洛爾組及參麥小大劑量組。五組大鼠均經腹腔註射給藥，于治療前及30 d 後分彆採用反轉錄-聚閤酶鏈反應（RT-PCR）和免疫組織化學檢測心肌 VEGF 和缺氧誘導因子-1a（HIF-1a）HIF-la 錶達情況、受體胎肝激酶1（FLK-1），籍此攷察參麥對大鼠急性心肌缺血血管內皮生長因子錶達的影響。結果：參麥明顯增高 VEGF 蛋白質及 mRNA、HIF-1a、FLK-1的錶達，縮小心肌缺血麵積，與模型組比較（P ＜0．05），差異均有統計學意義，參麥小、大劑量組間差異無統計學意義。結論：參麥可減輕大鼠急性心肌缺血缺氧性損傷和血管內皮炎癥反應程度，其作用機製可能是其促進大鼠缺血心肌血管內皮再生有關，對改善再灌註損傷血管內皮功能紊亂有一定的防治作用。
목적：관찰삼맥대대서급성심기결혈혈관내피생장인자표체적영향，탐토기작용궤제。방법：대서40지，채용결찰좌측관상동맥전강지30 min 후송해찰구재관주60 min，반복3차이조성심기결혈재관주손상모형，병수궤분위대조조、모형조、미탁락이조급삼맥소대제량조。오조대서균경복강주사급약，우치료전급30 d 후분별채용반전록-취합매련반응（RT-PCR）화면역조직화학검측심기 VEGF 화결양유도인자-1a（HIF-1a）HIF-la 표체정황、수체태간격매1（FLK-1），적차고찰삼맥대대서급성심기결혈혈관내피생장인자표체적영향。결과：삼맥명현증고 VEGF 단백질급 mRNA、HIF-1a、FLK-1적표체，축소심기결혈면적，여모형조비교（P ＜0．05），차이균유통계학의의，삼맥소、대제량조간차이무통계학의의。결론：삼맥가감경대서급성심기결혈결양성손상화혈관내피염증반응정도，기작용궤제가능시기촉진대서결혈심기혈관내피재생유관，대개선재관주손상혈관내피공능문란유일정적방치작용。
Objective:To observe the effect of Shenmai on expressions of myocardial vascular endothelial growth factors in rats with acute myocardial ischemia,and explore its mechanism.Methods:40 rats received ligation of left anterior descending coronary ar-tery.30 minutes later,release buckles reperfusion for 60 min to cause myocardial ischemia reperfusion injury model.The rats were then randomly divided into control group,model group,positive drug metoprolol group and Shenmai small dose,and Shenmai large dose group.Five groups of rats were treated with intraperitoneal injection,before treatment and 30 days respectively by reverse transcription polymerase chain reaction (RT-PCR)and immunohistochemical detection of myocardial VEGF and hypoxia inducible factor -1a (HIF-1a)HIF-la expression,receptor in fetal liver kinase 1 (FLK-1),the study of acute effect of nationality myocardi-al ischemic myocardium in rats of vascular endothelial growth factor expression.Results:Shenmai injection significantly increased the expression of VEGF protein and mRNA,HIF-1a,FLK-1,reduce the myocardial ischemic area,compared with the model group (P <0.05),the difference was statistically significant,no significant difference in small,large dose Shenmai group.Con-clusion:Shenmai injection can reduce the rat model of acute myocardial ischemia and hypoxia injury and vascular endothelial in-flammatory reaction.Its mechanism may be related to the vascular endothelial regeneration in rats with myocardial ischemia reper-fusion injury.It helps to improve the prevention of vascular endothelial dysfunction.
