安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
Acta Universitatis Medicinalis Anhui
2015年
10期
1497-1500
,共4页
杜世华%胡传玉%鲁大伟%李燕%桑琳
杜世華%鬍傳玉%魯大偉%李燕%桑琳
두세화%호전옥%로대위%리연%상림
吲哚胺2,3-二氧酶%子宫颈癌%CINⅢ%T淋巴细胞
吲哚胺2,3-二氧酶%子宮頸癌%CINⅢ%T淋巴細胞
신타알2,3-이양매%자궁경암%CINⅢ%T림파세포
indoleamine 2%3-dioxygenase%cervical cancer%CINⅢ%T lymphocytes
目的 研究吲哚胺2,3-二氧酶( IDO)表达对子宫颈癌患者T淋巴细胞活性的影响,进一步了解子宫颈癌的免疫逃逸机制. 方法 选择子宫颈上皮内瘤变Ⅲ级( CINⅢ)组织20例( CINⅢ组) ,子宫颈癌患者组织20 例(宫颈癌组) ,正常宫颈组织20例(正常组) ,采用流式细胞仪检测各组外周血中 CD3 + T 细胞、CD8 + T 细胞、CD4 + T 细胞、CD4 +CD25 + Foxp3 + Tregs数量、CD4 +T细胞/CD8 +T细胞. 采用Western blot 法和半定量 RT-PCR 法检测各组子宫颈组织IDO mRNA及其蛋白表达,分析各组子宫颈组织中IDO的表达对宿主T淋巴细胞活性的影响. 结果 正常组、CINⅢ组及宫颈癌组3组之间IDO蛋白表达强度差异有统计学意义(P<0. 001). 宫颈癌组IDO表达高于CINⅢ和正常组(P<0. 001),CINⅢ组高于正常组(P<0. 001). 宫颈癌组外周血中CD3 + T 细胞、CD8 + T 细胞、CD4 + T 细胞、CD4 + CD25 +Foxp3 +Tregs数量与正常组和CINⅢ组差异有统计学意义( P<0. 001). 3组中IDO蛋白表达与外周血中CD3 +T细胞、CD4 + T 细胞/CD8 + T 细胞呈负相关性,与 CD4 + CD25 +Foxp3 +Tregs呈正相关性. 结论 IDO抑制外周血T淋巴细胞对肿瘤的杀伤效应,使子宫颈癌患者免疫功能低下,有利于肿瘤细胞免疫逃逸,从而使病情进展.
目的 研究吲哚胺2,3-二氧酶( IDO)錶達對子宮頸癌患者T淋巴細胞活性的影響,進一步瞭解子宮頸癌的免疫逃逸機製. 方法 選擇子宮頸上皮內瘤變Ⅲ級( CINⅢ)組織20例( CINⅢ組) ,子宮頸癌患者組織20 例(宮頸癌組) ,正常宮頸組織20例(正常組) ,採用流式細胞儀檢測各組外週血中 CD3 + T 細胞、CD8 + T 細胞、CD4 + T 細胞、CD4 +CD25 + Foxp3 + Tregs數量、CD4 +T細胞/CD8 +T細胞. 採用Western blot 法和半定量 RT-PCR 法檢測各組子宮頸組織IDO mRNA及其蛋白錶達,分析各組子宮頸組織中IDO的錶達對宿主T淋巴細胞活性的影響. 結果 正常組、CINⅢ組及宮頸癌組3組之間IDO蛋白錶達彊度差異有統計學意義(P<0. 001). 宮頸癌組IDO錶達高于CINⅢ和正常組(P<0. 001),CINⅢ組高于正常組(P<0. 001). 宮頸癌組外週血中CD3 + T 細胞、CD8 + T 細胞、CD4 + T 細胞、CD4 + CD25 +Foxp3 +Tregs數量與正常組和CINⅢ組差異有統計學意義( P<0. 001). 3組中IDO蛋白錶達與外週血中CD3 +T細胞、CD4 + T 細胞/CD8 + T 細胞呈負相關性,與 CD4 + CD25 +Foxp3 +Tregs呈正相關性. 結論 IDO抑製外週血T淋巴細胞對腫瘤的殺傷效應,使子宮頸癌患者免疫功能低下,有利于腫瘤細胞免疫逃逸,從而使病情進展.
목적 연구신타알2,3-이양매( IDO)표체대자궁경암환자T림파세포활성적영향,진일보료해자궁경암적면역도일궤제. 방법 선택자궁경상피내류변Ⅲ급( CINⅢ)조직20례( CINⅢ조) ,자궁경암환자조직20 례(궁경암조) ,정상궁경조직20례(정상조) ,채용류식세포의검측각조외주혈중 CD3 + T 세포、CD8 + T 세포、CD4 + T 세포、CD4 +CD25 + Foxp3 + Tregs수량、CD4 +T세포/CD8 +T세포. 채용Western blot 법화반정량 RT-PCR 법검측각조자궁경조직IDO mRNA급기단백표체,분석각조자궁경조직중IDO적표체대숙주T림파세포활성적영향. 결과 정상조、CINⅢ조급궁경암조3조지간IDO단백표체강도차이유통계학의의(P<0. 001). 궁경암조IDO표체고우CINⅢ화정상조(P<0. 001),CINⅢ조고우정상조(P<0. 001). 궁경암조외주혈중CD3 + T 세포、CD8 + T 세포、CD4 + T 세포、CD4 + CD25 +Foxp3 +Tregs수량여정상조화CINⅢ조차이유통계학의의( P<0. 001). 3조중IDO단백표체여외주혈중CD3 +T세포、CD4 + T 세포/CD8 + T 세포정부상관성,여 CD4 + CD25 +Foxp3 +Tregs정정상관성. 결론 IDO억제외주혈T림파세포대종류적살상효응,사자궁경암환자면역공능저하,유리우종류세포면역도일,종이사병정진전.
Objective To investigate the expression of indoleamine2,3-dioxygenase(IDO) how to effect on T lym-phocyte activity, in order to understand the mechanism of immune escape of cervical cancer. Methods Selected 20 patients with CINⅢ,20 cases with cervical cancer and 20 cases with normal cervical for comparison, used flow cytometry to detcet the number of each group CD3 +T, CD4 +T, CD8 +T, CD4 +CD25 + Foxp3 +Tregs and CD4 +/CD8 +. Used semi-quantitative RT-PCR and Western blot to detect IDO mRNA and expression of IDO, and to ana-lyze the effection on T lymphocyte active in cervical tissue. Results The expression of IDO was significantly differ-ent among the three group(P<0. 001). The positive rate of IDO in cerival cancer was significantly higher than that in CINIII and normal cerival(P<0. 001). The positive rate of IDO in CINⅢ was significantly higher than that in normal cerival(P<0. 001). The number of CD3 +T, CD4 +T, CD8 +T and CD4 +CD25 +Foxp3 +Tregs was signifi-cantly different among the three group(P<0. 001). The positive expression of IDO was negatively correlated with the number of CD3 +T, CD4 +T, CD8 +T,and positively correlated with CD4 +CD25 +Foxp3 +Tregs. Conclusion IDO inhibits T lymphocyte activity. Cervical cancer patients with low immune function can cause the tumor cells es-cape form immune surveillance,may lead to disease progression.
