CAJ | 학술논문

目的：探讨脐带间充质干细胞（HUMSCs）移植对老年痴呆小鼠 SAMP8学习记忆能力的影响。方法：培养人脐带间充质干细胞，通过尾静脉以高中低3个剂量（2．5×105／0．2 mL／20 g、5×105／0．2 mL／20 g、1×106／0．2 mL／20 g）分别向7月龄 SAMP8老年痴呆小鼠移植 HUMSCs，并设立移植生理盐水组和 R1小鼠正常对照组。HUMSCs 移植45 d 后，MORRIS水迷宫和跳台实验检测其学习记忆能力。结果：与 R1正常对照小鼠比较，SAMP8老年痴呆小鼠生理盐水组学习记忆能力明显降低；和移植生理盐水组比较，细胞移植低剂量组水迷宫逃避潜伏期显著缩短（P ＜0．05），穿越平台次数显著增加（P ＜0．05）；跳台潜伏期显著延长（P ＜0．05），错误次数显著减少（P ＜0．05），结论：低剂量的脐带间充质干细胞移植能增强老年痴呆小鼠 SAMP8的学习记忆能力。
목적：탐토제대간충질간세포（HUMSCs）이식대노년치태소서 SAMP8학습기억능력적영향。방법：배양인제대간충질간세포，통과미정맥이고중저3개제량（2．5×105／0．2 mL／20 g、5×105／0．2 mL／20 g、1×106／0．2 mL／20 g）분별향7월령 SAMP8노년치태소서이식 HUMSCs，병설립이식생리염수조화 R1소서정상대조조。HUMSCs 이식45 d 후，MORRIS수미궁화도태실험검측기학습기억능력。결과：여 R1정상대조소서비교，SAMP8노년치태소서생리염수조학습기억능력명현강저；화이식생리염수조비교，세포이식저제량조수미궁도피잠복기현저축단（P ＜0．05），천월평태차수현저증가（P ＜0．05）；도태잠복기현저연장（P ＜0．05），착오차수현저감소（P ＜0．05），결론：저제량적제대간충질간세포이식능증강노년치태소서 SAMP8적학습기억능력。
Objective:To explore the effects of human umbilical cord mesenchymal stem cells(HUMSCSs)on the learning ability and memory of mice SAMP8 with Alzheimer’s disease.Methods:We cultivated human umbilical cord mesenchymal stem cells, provided three doses HUMSCs (2.5 ×105 /0.2 mL/20 g,5 ×105 /0.2 mL/20 g,1 ×106 /0.2 mL/20 g)for the transplantation of seven months SAMP8 Alzheimer’s mice by the tail vein,and set up the normal saline group and normal control group R1 mice for comparison.After 45 days,MORRIS water maze and jumping apparatus were used to test the ability of learning and memory of the subjects.Results:Compared with normal control mice R1,normal saline group of SAMP8 Alzheimer’s mice performed significant-ly worse on the learning and memory tests.But there was significant improvement in Morris water maze test compared with the nor-mal saline group,including shorter escape latency (P <0.05)more platform crossing frequencies (P <0.05),and in jumping apparatus including less jumping stand error number (P <0.05)and longer step down latency (P <0.05).Conclusion:Low dose of HUMSCs can enhance learning and memory ability of SAMP8 Alzheimer’s mice.