山东医药
山東醫藥
산동의약
Shandong Medical Journal
2015年
36期
21-23
,共3页
梗阻性黄疸%小肠黏膜%谷氨酰胺%蛋白激酶B%肠内营养%大鼠
梗阻性黃疸%小腸黏膜%穀氨酰胺%蛋白激酶B%腸內營養%大鼠
경조성황달%소장점막%곡안선알%단백격매B%장내영양%대서
obstructive jaundice%small intestine mucosa%glutamine%protein kinase B%enteral nutrition%rats
目的：观察梗阻性黄疸大鼠小肠组织中蛋白激酶 B （ Akt ）活性，探讨谷氨酰胺肠保护的作用机制。方法将64只SD大鼠随机分为4组各16只，模型组、甘氨酸组、谷氨酰胺组行梗阻性黄疸造模，假手术组游离上段胆总管后关腹；造模后1 d，甘氨酸组、谷氨酰胺组均给予同等热量的肠内营养剂灌胃，同时分别给予甘氨酸、谷氨酰胺1 g／（ kg· d），模型组、假手术组给予等量生理盐水灌胃。各组分别于术后第3、7天处死大鼠8只，采用Western blotting法检测小肠组织Akt总蛋白及p-Akt，实时PCR检测肠组织Akt mRNA。结果与假手术组比较，模型组、甘氨酸组、谷氨酰胺组术后第3、7天小肠组织中Akt mRNA及p-Akt蛋白增加，且谷氨酰胺组高于模型组、甘氨酸组，P均＜0．05；模型组、甘氨酸组间比较，P均＞0．05。各组小肠组织Akt总蛋白比较，P均＞0．05。结论谷氨酰胺通过促进Akt信号通路的活化，发挥对小肠上皮细胞的保护作用。
目的：觀察梗阻性黃疸大鼠小腸組織中蛋白激酶 B （ Akt ）活性，探討穀氨酰胺腸保護的作用機製。方法將64隻SD大鼠隨機分為4組各16隻，模型組、甘氨痠組、穀氨酰胺組行梗阻性黃疸造模，假手術組遊離上段膽總管後關腹；造模後1 d，甘氨痠組、穀氨酰胺組均給予同等熱量的腸內營養劑灌胃，同時分彆給予甘氨痠、穀氨酰胺1 g／（ kg· d），模型組、假手術組給予等量生理鹽水灌胃。各組分彆于術後第3、7天處死大鼠8隻，採用Western blotting法檢測小腸組織Akt總蛋白及p-Akt，實時PCR檢測腸組織Akt mRNA。結果與假手術組比較，模型組、甘氨痠組、穀氨酰胺組術後第3、7天小腸組織中Akt mRNA及p-Akt蛋白增加，且穀氨酰胺組高于模型組、甘氨痠組，P均＜0．05；模型組、甘氨痠組間比較，P均＞0．05。各組小腸組織Akt總蛋白比較，P均＞0．05。結論穀氨酰胺通過促進Akt信號通路的活化，髮揮對小腸上皮細胞的保護作用。
목적：관찰경조성황달대서소장조직중단백격매 B （ Akt ）활성，탐토곡안선알장보호적작용궤제。방법장64지SD대서수궤분위4조각16지，모형조、감안산조、곡안선알조행경조성황달조모，가수술조유리상단담총관후관복；조모후1 d，감안산조、곡안선알조균급여동등열량적장내영양제관위，동시분별급여감안산、곡안선알1 g／（ kg· d），모형조、가수술조급여등량생리염수관위。각조분별우술후제3、7천처사대서8지，채용Western blotting법검측소장조직Akt총단백급p-Akt，실시PCR검측장조직Akt mRNA。결과여가수술조비교，모형조、감안산조、곡안선알조술후제3、7천소장조직중Akt mRNA급p-Akt단백증가，차곡안선알조고우모형조、감안산조，P균＜0．05；모형조、감안산조간비교，P균＞0．05。각조소장조직Akt총단백비교，P균＞0．05。결론곡안선알통과촉진Akt신호통로적활화，발휘대소장상피세포적보호작용。
Objective To observe the activity changes of protein kinase B ( Akt) in the small intestine of rats with ob-structive jaundice, and to investigate the mechanism of glutamine in intestinal protection.Methods Sixty-four SD rats were randomly divided into four groups, 16 rats in each group.The obstructive jaundice models were made in the groups A, B and C.After freeing the upper common bile duct and then we closed the abdomen of rats in the group D.One day after modeling, rats in the groups B and C were given the same amount of heat enteral nutrition by gavage, and at the same time, the glycine and glutamine 1 g/( kg· d) was added.Rats in the groups A and D were given the same volume of normal saline by gavage. Eight rats were sacrificed at day 3 and 7 after surgery.Western blotting was used to detect the small intestine tissue’s total protein of Akt and p-Akt, RT-PCR method was used to detect Akt mRNA of intestine tissue.Results Compare with group D, the Akt mRNA and p-Akt protein was increased at day 3 and 7 after surgery in the groups A, B and C, and group C was higher than groups A and B (all P<0.05).No significant difference was found between group A and group B (all P>0.05).No sig-nificant difference was found in Akt total protein of small intestine among these groups (all P>0.05).Conclusion The gluta-mine protects the epithelial cells of small intestine by promoting the activation of Akt signaling pathway.
